Abstract
The fruit fly Drosophila melanogaster is one of the most widely used and well-studied model organisms in biology and therefore a promising tool for quantitative proteomics. Here, we describe a method to label D. melanogaster with stable isotope labeled amino acids in vivo. Feeding flies with heavy lysine labeled yeast cells leads to virtually complete heavy labeling already in the first filial generation. The approach is simple, fast, and cost-effective, which makes SILAC flies an attractive model system for the emerging field of in vivo quantitative proteomics.
Matthias D. Sury and Jia-Xuan Chen have equally contributed to this Chapter.
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Acknowledgements
We would like to thank Erik McShane for critical comments on the manuscript. This work was supported by the Helmholtz Association and the Swiss National Science Foundation.
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Sury, M.D., Chen, JX., Selbach, M. (2014). In Vivo Stable Isotope Labeling by Amino Acids in Drosophila melanogaster . In: Warscheid, B. (eds) Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC). Methods in Molecular Biology, vol 1188. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1142-4_7
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DOI: https://doi.org/10.1007/978-1-4939-1142-4_7
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