Abstract
Upon ligand binding, Notch activation and cleavage culminates in the expression of its target genes. Hence, the use of Notch-responsive promoters to drive reporter gene expression provides a flexible and robust approach for monitoring signaling activity. In this chapter, we present an overview of Notch transcriptional reporter assays and discuss different methods for ligand-independent and ligand-dependent activation of Notch in mammalian cells.
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Acknowledgements
We thank members of the Kopan lab for technical assistance and helpful discussions, most especially to Scott Boyle for providing the fluorescence images of the CHO co-cultures and to Matt Hass for reading the manuscript. This work was supported by NIH grant GM55479.
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Ilagan, M.X.G., Kopan, R. (2014). Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays. In: Bellen, H., Yamamoto, S. (eds) Notch Signaling. Methods in Molecular Biology, vol 1187. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1139-4_11
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DOI: https://doi.org/10.1007/978-1-4939-1139-4_11
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