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Gene Expression Profiling of Hematopoietic Stem Cells (HSCs)

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Hematopoietic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1185))

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Abstract

Transcriptomic analysis to decipher the molecular phenotype of hematopoietic stem cells, regulatory mechanisms directing their life cycle, and the molecular signals mediating proliferation, mobilization, migration, and differentiation is believed to unravel disease-specific disturbances in hematological diseases and assist in the development of novel cell-based clinical therapies in this era of genomic medicine. The recent advent in genomic tools and technologies is now enabling the study of such comprehensive transcriptional characterization of cell types in a robust and successful manner. This chapter describes detailed protocols for isolating RNA from purified population of hematopoietic cells and gene expression profiling of those purified cells using both microarrays (Affymetrix) and RNA-Seq technology (Illumina Platform).

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Acknowledgements

The author gratefully acknowledges the Genomics Core Facility in intramural research in NHLBI and the extramural research program in NIA for their support and resources during the preparation of this chapter.

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Authors and Affiliations

  1. Division of Geriatrics and Clinical Gerontology, National Institute on Aging, Gateway Building, Suite 3C307, 7201 Wisconsin Avenue, Bethesda, MD, 20892-9205, USA

    Nalini Raghavachari

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  1. Nalini Raghavachari
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Correspondence to Nalini Raghavachari .

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Editors and Affiliations

  1. Department of Pediatrics, Aflac Cancer and Blood Disorders Center Emory University School of Medicine, Atlanta, Georgia, USA

    Kevin D. Bunting

  2. Department of Pediatrics, Emory University School of Medicine Aflac Cancer & Blood Disorders Ctr, Atlanta, Georgia, USA

    Cheng-Kui Qu

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Raghavachari, N. (2014). Gene Expression Profiling of Hematopoietic Stem Cells (HSCs). In: Bunting, K., Qu, CK. (eds) Hematopoietic Stem Cell Protocols. Methods in Molecular Biology, vol 1185. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1133-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-1133-2_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1132-5

  • Online ISBN: 978-1-4939-1133-2

  • eBook Packages: Springer Protocols

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