Abstract
Lentiviral vectors can be used to genetically modify a broad range of cells. Hematopoietic stem cells (HSCs) are particularly suitable for lentiviral gene augmentation, because these cells can be enriched with relative ease from mouse bone marrow and human hematopoietic sources, and in principle require relatively limited cell numbers to completely reconstitute the hematopoietic system in vivo. Furthermore, lentiviral vectors are very efficient if pseudotyped with broad tropism envelope proteins. This chapter focuses on gene modification by the use of self-inactivating third-generation human immunodeficiency virus-derived lentiviral vectors for ex vivo HSC modification for both mouse and human application.
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Acknowledgements
This work was supported by the European Commission’s 5th, 6th, and 7th Framework Programs, Contracts QLK3-CT-2001-00427-INHERINET, LSHB-CT-2004-005242-CONSERT, 222878-PERSIST, and 261387-CELL-PID, and by the Netherlands Organization for Health Research ZonMW, program grants 431-00-016 and 434-00-010.
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van Til, N.P., Wagemaker, G. (2014). Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells. In: Bunting, K., Qu, CK. (eds) Hematopoietic Stem Cell Protocols. Methods in Molecular Biology, vol 1185. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1133-2_21
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DOI: https://doi.org/10.1007/978-1-4939-1133-2_21
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