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Five-Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells

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Hematopoietic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1185))

Abstract

Hematopoietic stem cells (HSCs) have self-renewal activity and multipotency. Clonal analysis and determination of HSC differentiation potential into platelets and erythrocytes as well as leukocytes are essential for the study of self-renewal and lineage commitment in HSC. However, due to technical limitations, platelet and erythrocyte differentiation potentials have not been assessed. This chapter describes principles and methods for single-cell sorting, single-cell transplantation, and identification and quantitative analysis of cell contribution to platelets and erythrocytes in addition to leukocytes in mouse chimeras.

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References

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Author information

Authors and Affiliations

  1. Department of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan

    Ryo Yamamoto, Yohei Morita & Hiromitsu Nakauchi

Authors
  1. Ryo Yamamoto
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  2. Yohei Morita
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  3. Hiromitsu Nakauchi
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Corresponding author

Correspondence to Hiromitsu Nakauchi .

Editor information

Editors and Affiliations

  1. Department of Pediatrics, Aflac Cancer and Blood Disorders Center Emory University School of Medicine, Atlanta, Georgia, USA

    Kevin D. Bunting

  2. Department of Pediatrics, Emory University School of Medicine Aflac Cancer & Blood Disorders Ctr, Atlanta, Georgia, USA

    Cheng-Kui Qu

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© 2014 Springer Science+Business Media New York

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Yamamoto, R., Morita, Y., Nakauchi, H. (2014). Five-Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells. In: Bunting, K., Qu, CK. (eds) Hematopoietic Stem Cell Protocols. Methods in Molecular Biology, vol 1185. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1133-2_16

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  • DOI: https://doi.org/10.1007/978-1-4939-1133-2_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1132-5

  • Online ISBN: 978-1-4939-1133-2

  • eBook Packages: Springer Protocols

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