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Whole-Cell Voltage Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique

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Patch-Clamp Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1183))

Abstract

Control of membrane voltage and membrane current measurements are of critical importance for the study of numerous aspects of skeletal muscle physiology and pathophysiology. The silicone-clamp technique makes use of a conventional patch-clamp apparatus to achieve whole-cell voltage clamp of a restricted portion of a fully differentiated adult skeletal muscle fiber. The major part of an isolated muscle fiber is insulated from the extracellular medium with silicone grease and the tip of a single microelectrode connected to the amplifier is then inserted within the fiber through the silicone layer. The method is extremely easy to implement. It represents an alternative to the traditional vaseline-gap isolation and two or three microelectrodes voltage-clamp techniques. The present chapter reviews the benefits of the silicone-clamp technique and provides updated detailed insights into its practical implementation.

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Acknowledgements

This work was supported by the Centre National de la Recherche Scientifique, the Université Claude Bernard Lyon 1, and the Association Française contre les Myopathies.

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Authors and Affiliations

  1. Centre de Génétique et de Physiologie Moléculaire et Cellulaire, UMR CNRS 5534, Université Claude Bernard - Lyon 1, Bât. Raphael Dubois, 43 boulevard du 11 novembre 1918, Villeurbanne Cedex, 69622, France

    Romain Lefebvre, Bruno Allard & Vincent Jacquemond

  2. CNRS UMR 5297, Interdisciplinary Institute for Neuroscience, University of Bordeaux, 146 rue Léo-Saignat, Bordeaux Cedex, 33077, France

    Romain Lefebvre

  3. CNRS UMR 5297, Interdisciplinary Institute for Neuroscience, University of Bordeaux, 146 rue Léo-Saignat, Bordeaux Cedex, 33077, France

    Sandrine Pouvreau

  4. UR406 Abeilles et Environnement, INRA, Domaine Saint Paul, Site Agroparc, CS 40 509, Avignon Cedex, 84914, France

    Sandrine Pouvreau & Claude Collet

Authors
  1. Romain Lefebvre
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  2. Sandrine Pouvreau
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  3. Claude Collet
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  4. Bruno Allard
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  5. Vincent Jacquemond
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Corresponding author

Correspondence to Vincent Jacquemond .

Editor information

Editors and Affiliations

  1. Department of Translational Bioscience, National Research Council of Canada, Ottawa, Ontario, Canada

    Marzia Martina

  2. Dept of Neuroscience & Brain Technologies, Italian Institute of Technology, Genoa, Italy

    Stefano Taverna

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Lefebvre, R., Pouvreau, S., Collet, C., Allard, B., Jacquemond, V. (2014). Whole-Cell Voltage Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique. In: Martina, M., Taverna, S. (eds) Patch-Clamp Methods and Protocols. Methods in Molecular Biology, vol 1183. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1096-0_9

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  • DOI: https://doi.org/10.1007/978-1-4939-1096-0_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1095-3

  • Online ISBN: 978-1-4939-1096-0

  • eBook Packages: Springer Protocols

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