Abstract
The RNA electrophoretic mobility shift assay is a very versatile method to study a broad spectrum of RNA–protein interactions. The technique is based on the separation of protein RNA mixtures through a native acrylamide gel. Compared to unbound RNA, RNA–protein complexes migrate slower through the gel resulting in a mass shift. The RNA EMSA can be used to identify unknown RNA–protein complexes, to map the RNA binding site of single proteins or determine the specificity of RNA–protein complexes using specific antibodies resulting in retarded migration through the gel.
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Reboll, M.R. (2014). Mapping of Protein Binding RNA Elements. In: Alvarez, M., Nourbakhsh, M. (eds) RNA Mapping. Methods in Molecular Biology, vol 1182. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1062-5_16
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DOI: https://doi.org/10.1007/978-1-4939-1062-5_16
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