Abstract
Protein engineering by random circular permutation is an effective tool for tailoring protein topology with potential functional benefits including improved catalytic activity. This method involves covalently connecting the native protein termini with a peptide linker and cleaving a peptide bond elsewhere in the polypeptide sequence. Termini relocation can impact protein ternary and quaternary structure and translate into functional enhancements due to changes in protein conformation and flexibility. As the effects of new termini in specific protein locations are difficult to predict, the preparation of a library constituting all possible permutation sites is an effective search strategy for identifying variants with novel properties.
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Acknowledgments
The authors like to thank the members of the Lutz Lab for many helpful comments and suggestions on the manuscript. This work was in part supported by funds from the US National Science Foundation (CBET 0730312 & 1159434).
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Lutz, S., Daugherty, A.B., Yu, Y., Qian, Z. (2014). Generating Random Circular Permutation Libraries. In: Gillam, E., Copp, J., Ackerley, D. (eds) Directed Evolution Library Creation. Methods in Molecular Biology, vol 1179. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1053-3_17
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DOI: https://doi.org/10.1007/978-1-4939-1053-3_17
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