Skip to main content
SpringerLink
Log in

Liver: Tissue Handling and Evaluation

  • Protocol
  • First Online:
Histopathology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1180))

Abstract

Liver diseases remain among the most important causes of morbidity and mortality worldwide. Although the diagnostic tools (e.g., blood studies, imaging, genetic and molecular tests) available to clinicians have greatly expanded in number and increased in sensitivity, the examination of liver tissue by a pathologist skilled and experienced in hepatopathology remains vitally important in the evaluation and care of the patient with liver abnormalities. In some disorders, such as autoimmune hepatitis, liver biopsy is considered mandatory. The indications for performing liver biopsies have changed over the years (e.g., large duct obstruction was a common diagnostic problem 50 years ago and is only uncommonly so currently). Liver samples come to the pathologist as aspiration biopsies for cytologic examination, tissue biopsies (fine needle, core, transjugular, and wedge), resections, and explants. Each demands slightly different approaches for optimal handling and evaluation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Kanel GC, Korula J (2005) Atlas of liver pathology, 2nd edn. Elsevier Saunders, Philadelphia, PA

    Google Scholar 

  2. Geller SA, Petrovic LM (2009) Biopsy interpretation of the liver, 2nd edn. Lippincott Williams & Wilkins, Philadelphia, PA

    Google Scholar 

  3. Lefkowitch JH (2010) Scheuer’s liver biopsy interpretation, 8th edn. Elsevier Saunders, Philadelphia, PA

    Google Scholar 

  4. Saxena R (2011) Practical hepatic pathology. Elsevier Saunders, Philadelphia, PA

    Google Scholar 

  5. Burt AD, Portmann B, Ferrell L (2012) MacSween’s pathology of the liver, 6th edn. Churchill Livingstone, Edinburgh

    Google Scholar 

  6. Bell LN, Theodorakis JL, Vuppalanchi R et al (2010) Serum proteomics and biomarker discovery across the spectrum of nonalcoholic fatty liver disease. Hepatology 51:111–120

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Lim HY, Sohn I, Deng S et al (2013) Prediction of disease-free survival in hepatocellular carcinoma by gene expression profiling. Ann Surg Oncol 20:3747–3753

    Article  PubMed  Google Scholar 

  8. Moylan CA, Pang H, Delinger A et al (2014) Hepatic gene expression profiles differentiate pre-symptomatic patients with mild versus severed nonalcoholic fatty liver disease (Severe NAFLD Gene Signature). Hepatology 59(2):471–482. doi:10.1002/hep.26661

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Paradis V, Degos F, Dargère D et al (2005) Identification of a new marker of hepatocellular carcinoma by serum protein profiling of patients with chronic liver diseases. Hepatology 41:40–47

    Article  CAS  PubMed  Google Scholar 

  10. Revill K, Wang T, Lachenmayer A et al (2013) Genome-wide methylation analysis and epigenetic unmasking identify tumor suppressor genes in hepatocellular carcinoma. Gastroenterology 145:1424–1435

    Article  CAS  PubMed  Google Scholar 

  11. Smallin RL, Delker DA, Zhang Y et al (2013) Genome-wide transcriptome analysis identifies novel gene signatures implicated in chronic liver disease. Am J Physiol Gastrointest Liver Physiol 305:G364–G374

    Article  Google Scholar 

  12. Uto H, Kanmura S, Takami Y, Tsubouchi H (2010) Clinical proteomics for liver disease: a promising approach for discovery of novel biomarkers. Proteome Sci 8:70. doi:10.1186/1477-5956-8-70

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Zhang L, Ma D, Deng C et al (2013). Gene expression profiles of peripheral blood mononuclear cells in primary biliary cirrhosis. Clin Exp Med, epub ahead of print

    Google Scholar 

  14. Perkins EM, McCaffery JM (2007) Conventional and immunoelectron microscopy of mitochondria. Methods Mol Biol 372:467–483

    Article  CAS  PubMed  Google Scholar 

  15. Kiernan J (2008) Histologic and histochemical methods: theory and practice, 4th edn. Scion, Bloxham Mill

    Google Scholar 

  16. Carson FL, Hladik C (2009) Histotechnology – a self-instructional text, 3rd edn. American Society of Clinical Pathology Press, Chicago, IL

    Google Scholar 

  17. Suvarna KS, Layton C, Bancroft JD (2013) Bancroft’s theory and practice of histological techniques. Churchill Livingstone, London

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at University of California, Los Angeles, CA, USA

    Stephen A. Geller

  2. Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY, USA

    Stephen A. Geller

Authors
  1. Stephen A. Geller
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Stephen A. Geller .

Editor information

Editors and Affiliations

  1. Department of Pathology, USC Keck School of Medicine, Los Angeles, California, USA

    Christina E. Day

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media, New York

About this protocol

Cite this protocol

Geller, S.A. (2014). Liver: Tissue Handling and Evaluation. In: Day, C. (eds) Histopathology. Methods in Molecular Biology, vol 1180. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1050-2_18

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-1050-2_18

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1049-6

  • Online ISBN: 978-1-4939-1050-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation