Abstract
Micropatterning is a powerful technique to control cell shape and position on a culture substrate. In this chapter, we describe the method to reproducibly create large numbers of micropatterned heterotypic cell pairs with defined size, shape, and length of cell–cell contact. These cell pairs can be utilized in patch clamp recordings to quantify electrical interactions between cardiomyocytes and non-cardiomyocytes.
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Acknowledgements
We thank Dr. Robert Kirkton for reading of the manuscript. This work was supported by the National Heart, Lung, and Blood Institute of the NIH under Award Numbers R01-HL-104326 and R21-HL-106203. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.
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Nguyen, H., Badie, N., McSpadden, L., Pedrotty, D., Bursac, N. (2014). Quantifying Electrical Interactions Between Cardiomyocytes and Other Cells in Micropatterned Cell Pairs. In: Radisic, M., Black III, L. (eds) Cardiac Tissue Engineering. Methods in Molecular Biology, vol 1181. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1047-2_21
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DOI: https://doi.org/10.1007/978-1-4939-1047-2_21
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