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Cardiac Tissue Engineering pp 189–202Cite as

Preparation of Acellular Myocardial Scaffolds with Well-Preserved Cardiomyocyte Lacunae, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1181))

Abstract

Cardiac tissue engineering/regeneration using decellularized myocardium has attracted great research attention due to its potential benefit for myocardial infarction (MI) treatment. Here we describe an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae and a multi-stimulation bioreactor that is able to provide coordinated mechanical and electrical stimulation for facilitating cardiac construct development.

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Acknowledgments

This study is supported by NIH National Heart, Lung, and Blood Institute grant HL097321. The authors also would like to acknowledge the support from American Heart Association (13GRNT17150041) and MAFES Strategic Research Initiative (CRESS MIS-361020).

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Authors and Affiliations

  1. Tissue Bioengineering Laboratory, Department of Biological Engineering, Mississippi State University, 130 Creelman Street, Room 222, Mississippi State, MS, 39762, USA

    Bo Wang, Lakiesha N. Williams & Jun Liao Ph.D.

  2. Department of Surgery, Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA

    Bo Wang

  3. Department of Biomedical Engineering, University of Memphis, Memphis, TN, 38152, USA

    Amy L. de Jongh Curry

Authors
  1. Bo Wang
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  2. Lakiesha N. Williams
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  3. Amy L. de Jongh Curry
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  4. Jun Liao Ph.D.
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Corresponding author

Correspondence to Jun Liao Ph.D. .

Editor information

Editors and Affiliations

  1. Department Chemical Engineering&Applied Chemistry, University of Toronto, Toronto, Ontario, Canada

    Milica Radisic

  2. Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA

    Lauren D. Black III

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Wang, B., Williams, L.N., de Jongh Curry, A.L., Liao, J. (2014). Preparation of Acellular Myocardial Scaffolds with Well-Preserved Cardiomyocyte Lacunae, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct. In: Radisic, M., Black III, L. (eds) Cardiac Tissue Engineering. Methods in Molecular Biology, vol 1181. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1047-2_17

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  • DOI: https://doi.org/10.1007/978-1-4939-1047-2_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1046-5

  • Online ISBN: 978-1-4939-1047-2

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