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Protein Affinity Tags pp 35–45Cite as

Purification of a Recombinant Protein with Cellulose-Binding Module 3 as the Affinity Tag

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1177))

Abstract

Easy-to-perform and low-cost protein purification methods are in high demand for the mass production of commonly used enzymes that play an important role in bioeconomy. A low-cost and rapid recombinant protein purification system was developed using CBM3 (family 3 cellulose-binding module) as affinity tag. This protocol describes the purification of CBM3-fusion protein and tag-free protein expressed in Pichia pastoris using CBM3 as an affinity tag.

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Acknowledgments

This work was supported by a grant-in-aid from the Scientific Research Foundation for Returned Scholars, Ministry of Education of China, the Fundamental Research Funds for the Central Universities (No. KA2070000008), and the National Basic Research Program of China (No. 2011CBA00801).

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Authors and Affiliations

  1. School of Life Science, University of Science and Technology of China, Huangshan Road 443, 230026, Hefei, Anhui, People’s Republic of China

    Dongmei Wang & Jiong Hong

Authors
  1. Dongmei Wang
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  2. Jiong Hong
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Corresponding author

Correspondence to Jiong Hong .

Editor information

Editors and Affiliations

  1. Organic & Biological Mass Spectrometry, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

    Richard J. Giannone

  2. Analytical Sciences, Amgen, West Greenwich, Rhode Island, USA

    Andrew B. Dykstra

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Wang, D., Hong, J. (2014). Purification of a Recombinant Protein with Cellulose-Binding Module 3 as the Affinity Tag. In: Giannone, R., Dykstra, A. (eds) Protein Affinity Tags. Methods in Molecular Biology, vol 1177. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1034-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-1034-2_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1033-5

  • Online ISBN: 978-1-4939-1034-2

  • eBook Packages: Springer Protocols

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