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Eosinophils pp 81-92 | Cite as

Identification of Human Eosinophils in Whole Blood by Flow Cytometry

  • Caroline Ethier
  • Paige Lacy
  • Francis DavoineEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)

Abstract

Identification of eosinophils in whole blood samples by flow cytometry is often problematic. There are usually only a low number and percentage of cells that may be detected, and it may be difficult to discriminate eosinophils from other granulocytes. Here, we propose a simple approach using the eosinophil’s intrinsic autofluorescence properties, combined with detection of CCR3 expression, to reliably identify eosinophils in a mixed leukocyte population.

Key words

Flow cytometry Eosinophils Granulocytes White blood cell differential CCR3 CD193 

Notes

Acknowledgements

This work was supported by funding from Agriculture Life and Environmental Science (ALES) and Campus Saint-Jean research funds from University of Alberta to FD.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Pulmonary Research Group, Department of MedicineUniversity of AlbertaEdmontonCanada
  2. 2.Campus Saint-JeanUniversity of AlbertaEdmontonCanada
  3. 3.Pulmonary Research GroupUniversity of AlbertaEdmontonCanada

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