Identification of Human Eosinophils in Whole Blood by Flow Cytometry
Identification of eosinophils in whole blood samples by flow cytometry is often problematic. There are usually only a low number and percentage of cells that may be detected, and it may be difficult to discriminate eosinophils from other granulocytes. Here, we propose a simple approach using the eosinophil’s intrinsic autofluorescence properties, combined with detection of CCR3 expression, to reliably identify eosinophils in a mixed leukocyte population.
Key wordsFlow cytometry Eosinophils Granulocytes White blood cell differential CCR3 CD193
This work was supported by funding from Agriculture Life and Environmental Science (ALES) and Campus Saint-Jean research funds from University of Alberta to FD.