Eosinophils pp 81-92 | Cite as

Identification of Human Eosinophils in Whole Blood by Flow Cytometry

  • Caroline Ethier
  • Paige Lacy
  • Francis DavoineEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)


Identification of eosinophils in whole blood samples by flow cytometry is often problematic. There are usually only a low number and percentage of cells that may be detected, and it may be difficult to discriminate eosinophils from other granulocytes. Here, we propose a simple approach using the eosinophil’s intrinsic autofluorescence properties, combined with detection of CCR3 expression, to reliably identify eosinophils in a mixed leukocyte population.

Key words

Flow cytometry Eosinophils Granulocytes White blood cell differential CCR3 CD193 



This work was supported by funding from Agriculture Life and Environmental Science (ALES) and Campus Saint-Jean research funds from University of Alberta to FD.


  1. 1.
    Hartnell A, Moqbel R, Walsh GM, Bradley B, Kay AB (1990) Fc gamma and CD11/CD18 receptor expression on normal density and low density human eosinophils. Immunology 69:264–270PubMedCentralPubMedGoogle Scholar
  2. 2.
    Davoine F, Labonte I, Ferland C, Mazer B, Chakir J, Laviolette M (2004) Role and modulation of CD16 expression on eosinophils by cytokines and immune complexes. Int Arch Allergy Immunol 134:165–172PubMedCrossRefGoogle Scholar
  3. 3.
    Davoine F, Lavigne S, Chakir J, Ferland C, Boulay ME, Laviolette M (2002) Expression of FcgammaRIII (CD16) on human peripheral blood eosinophils increases in allergic conditions. J Allergy Clin Immunol 109:463–469PubMedCrossRefGoogle Scholar
  4. 4.
    Valerius T, Repp R, Kalden JR, Platzer E (1990) Effects of IFN on human eosinophils in comparison with other cytokines. A novel class of eosinophil activators with delayed onset of action. J Immunol 145:2950–2958PubMedGoogle Scholar
  5. 5.
    Zhu X, Hamann KJ, Munoz NM, Rubio N, Mayer D, Hernrreiter A, Leff AR (1998) Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human eosinophils. J Immunol 161:2574–2579PubMedGoogle Scholar
  6. 6.
    Hartnell A, Kay AB, Wardlaw AJ (1992) IFN-gamma induces expression of Fc gamma RIII (CD16) on human eosinophils. J Immunol 148:1471–1478PubMedGoogle Scholar
  7. 7.
    Lavigne S, Bosse M, Boulet LP, Laviolette M (1997) Identification and analysis of eosinophils by flow cytometry using the depolarized side scatter-saponin method. Cytometry 29:197–203PubMedCrossRefGoogle Scholar
  8. 8.
    Thurau AM, Schylz U, Wolf V, Krug N, Schauer U (1996) Identification of eosinophils by flow cytometry. Cytometry 23:150–158PubMedCrossRefGoogle Scholar
  9. 9.
    Faucher JL, Lacronique-Gazaille C, Frebet E, Trimoreau F, Donnard M, Bordessoule D, Lacombe F, Feuillard J (2007) “6 markers/5 colors” extended white blood cell differential by flow cytometry. Cytometry A 71:934–944PubMedCrossRefGoogle Scholar
  10. 10.
    Ponath PD, Qin S, Post TW, Wang J, Wu L, Gerard NP, Newman W, Gerard C, Mackay CR (1996) Molecular cloning and characterization of a human eotaxin receptor expressed selectively on eosinophils. J Exp Med 183:2437–2448PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Pulmonary Research Group, Department of MedicineUniversity of AlbertaEdmontonCanada
  2. 2.Campus Saint-JeanUniversity of AlbertaEdmontonCanada
  3. 3.Pulmonary Research GroupUniversity of AlbertaEdmontonCanada

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