Eosinophils pp 45-51 | Cite as

Eosinophil Cell Lines

  • Kenji IshiharaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)


Eosinophilic cell lines, HL-60 clone 15 cells and EoL-1 cells, have contributed to clarifying the mechanisms responsible for differentiation into eosinophils. These cells differentiate into eosinophils by continuous histone acetylation. Histone deacetylase inhibitors, sodium butyrate and apicidin, promote the transactivation of various genes in these cells by causing the hyperacetylation of histones, resulting in the differentiation of cells into eosinophils. In contrast, transient acetylation by histone deacetylase inhibitors such as trichostatin A does not induce eosinophilic differentiation. This chapter describes the maintenance of HL-60 clone 15 cells and EoL-1 cells and induction of the differentiation of these cell lines into eosinophils by the histone deacetylase inhibitor sodium butyrate.

Key words

Eosinophils Eosinophilic cell line HL-60 clone 15 cells EoL-1 cells Sodium butyrate Histone Acetylation 



This work was supported by a grant-in-aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) (23791098).


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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Laboratory of Medical Science, Course for School Nurse Teacher, Faculty of EducationIbaraki UniversityMitoJapan

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