Advertisement

Eosinophils pp 45-51 | Cite as

Eosinophil Cell Lines

  • Kenji IshiharaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)

Abstract

Eosinophilic cell lines, HL-60 clone 15 cells and EoL-1 cells, have contributed to clarifying the mechanisms responsible for differentiation into eosinophils. These cells differentiate into eosinophils by continuous histone acetylation. Histone deacetylase inhibitors, sodium butyrate and apicidin, promote the transactivation of various genes in these cells by causing the hyperacetylation of histones, resulting in the differentiation of cells into eosinophils. In contrast, transient acetylation by histone deacetylase inhibitors such as trichostatin A does not induce eosinophilic differentiation. This chapter describes the maintenance of HL-60 clone 15 cells and EoL-1 cells and induction of the differentiation of these cell lines into eosinophils by the histone deacetylase inhibitor sodium butyrate.

Key words

Eosinophils Eosinophilic cell line HL-60 clone 15 cells EoL-1 cells Sodium butyrate Histone Acetylation 

Notes

Acknowledgement

This work was supported by a grant-in-aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) (23791098).

References

  1. 1.
    Fischkoff SA, Pollak A, Gleich GJ, Testa JR, Misawa S, Reber TJ (1984) Eosinophilic differentiation of the human promyelocytic leukemia cell line, HL-60. J Exp Med 160:179–196PubMedCrossRefGoogle Scholar
  2. 2.
    Fischkoff SA, Condon ME (1985) Switch in differentiative response to maturation inducers of human promyelocytic leukemia cells by prior exposure to alkaline conditions. Cancer Res 45: 2065–2069PubMedGoogle Scholar
  3. 3.
    Fischkoff SA (1988) Graded increase in probability of eosinophilic differentiation of HL-60 promyelocytic leukemia cells induced by culture under alkaline conditions. Leuk Res 12:679–686PubMedCrossRefGoogle Scholar
  4. 4.
    Saito H, Bourinbaiar A, Ginsburg M, Minato K, Ceresi E, Yamada K, Machover D, Bréard J, Mathé G (1985) Establishment and characterization of a new human eosinophilic leukemia cell line. Blood 66:1233–1240PubMedGoogle Scholar
  5. 5.
    Ishihara K, Hong J, Zee O, Ohuchi K (2004) Possible mechanism of action of the histone deacetylase inhibitors for the induction of differentiation of HL-60 clone 15 cells into eosinophils. Br J Pharmacol 142:1020–1030PubMedCentralPubMedCrossRefGoogle Scholar
  6. 6.
    Ishihara K, Takahashi A, Kaneko M, Sugeno H, Hirasawa N, Hong J, Zee O, Ohuchi K (2007) Differentiation of eosinophilic leukemia EoL-1 cells into eosinophils induced by histone deacetylase inhibitors. Life Sci 80:1213–1220PubMedCrossRefGoogle Scholar
  7. 7.
    Cools J, DeAngelo DJ, Gotlib J, Stover EH, Legare RD, Cortes J, Kutok J, Clark J, Galinsky I, Griffin JD, Cross NC, Tefferi A, Malone J, Alam R, Schrier SL, Schmid J, Rose M, Vandenberghe P, Verhoef G, Boogaerts M, Wlodarska I, Kantarjian H, Marynen P, Coutre SE, Stone R, Gilliland DG (2003) A tyrosine kinase created by fusion of the PDGFRA and FIP1L1 genes as a therapeutic target of imatinib in idiopathic hypereosinophilic syndrome. N Engl J Med 348:1201–1214PubMedCrossRefGoogle Scholar
  8. 8.
    Griffin JH, Leung J, Bruner RJ, Caligiuri MA, Briesewitz R (2003) Discovery of a fusion kinase in EOL-1 cells and idiopathic hypereosinophilic syndrome. Proc Natl Acad Sci U S A 100:7830–7835PubMedCentralPubMedCrossRefGoogle Scholar
  9. 9.
    Cools J, Quentmeier H, Huntly BJ, Marynen P, Griffin JD, Drexler HG, Gilliland DG (2004) The EOL-1 cell line as an in vitro model for the study of FIP1L1-PDGFRA-positive chronic eosinophilic leukemia. Blood 103:2802–2805PubMedCrossRefGoogle Scholar
  10. 10.
    Gotlib J, Cools J (2008) Five years since the discovery of FIP1L1-PDGFRA: what we have learned about the fusion and other molecularly defined eosinophilias. Leukemia 22:1999–2010PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Laboratory of Medical Science, Course for School Nurse Teacher, Faculty of EducationIbaraki UniversityMitoJapan

Personalised recommendations