Several studies suggest that eosinophil progenitor cells are capable of extramedullary proliferation but also enhance chronic inflammation via their own production of inflammatory and chemotactic mediators, thus augmenting the degree of inflammation by recruitment of more progenitors or mature effector cells, such as eosinophils at the site of inflammation. In this chapter, we provide methods focused on detecting eosinophil progenitor cells in the lung of allergen-challenged mice and how to monitor their proliferation capacity.
Allergy Asthma Bone marrow Lung Eosinophil progenitors In situ hematopoiesis CD34 IL-5Ra CCR3 Fluorescence-activated cell (FACS)
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The authors would like to thank Dr. Carina Malmhäll and Dr. You Lu, Krefting Research Centre, Gothenburg University for participating in the improvement and optimization of the Flow Cytometry protocols used at Krefting Research Centre and the generation of the original data showed in the figures.
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