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Eosinophils pp 21-27 | Cite as

Eosinophil Purification from Human Bone Marrow

  • Tina W. Wong
  • Diane F. JelinekEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)

Abstract

Eosinophils are innate immune cells that are best known for their involvement in host defense against parasitic infections and in asthma and allergic diseases. In vitro characterization of the function of human eosinophils has traditionally relied on the purification of these cells from the peripheral blood as reviewed in Chapter 2. Here, we describe a newly developed protocol for the purification of eosinophils from human bone marrow.

Key words

Bone marrow eosinophils Purification Human bone marrow Cell isolation 

Notes

Acknowledgments

This work was supported by the NIH Predoctoral Immunology Training Grant (T32 AI07425). TWW thanks the Mayo Medical School, Mayo Graduate School, and the Mayo Clinic Medical Scientist Training Program for providing an outstanding training environment.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Department of ImmunologyMayo ClinicRochesterUSA

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