Eosinophils pp 283-293 | Cite as

Eosinophils and the Ovalbumin Mouse Model of Asthma

  • F. Daubeuf
  • Nelly FrossardEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)


Mouse models of asthma are essential to understand asthma pathogenesis and eosinophil recruitment in the airways, and to develop new therapeutic strategies. Animal models try to mimic features of the human disease including airway hyperresponsiveness (AHR), eosinophilic inflammation, and remodeling, which are the typical asthma-related characteristics. The mouse is now the species of choice for asthma research due to the availability of transgenic animals and a wide array of specific reagents and techniques available. Cellular responses may be studied with innovative imaging and flow cytometry methods while lung mechanics may be precisely measured by the forced oscillation technique, and airway responsiveness approached by barometric plethysmography in conscious and unconstrained animals. Here, we describe procedures to generate acute models of hypereosinophilic asthma in mice, with ovalbumin (OVA) as the allergen. The presented allergic asthma models offer a large and reproducible eosinophil recruitment, measured in the bronchoalveolar lavage (BAL), accompanied with AHR, inflammation, and remodeling, and are particularly suited to assess the activity of drug candidates. We here present the classical 21-day allergic asthma model to OVA, and adjustments for a rapid 8-day model of airway allergic hypereosinophilia, and a more chronic 57-day model suitable for C57BL/6 mice to develop AHR together with airway eosinophilic inflammation and remodeling.

Key words

Allergic asthma Mouse model Ovalbumin Airway eosinophilia 



This work was supported by the “Fonds de Dotation Recherche en Santé Respiratoire, Appel d'Offres Blanc 2010.” FD is a recipient of a fellowship from the Laboratory of Excellence Medalis Initiative of Excellence (IdEx), Strasbourg University, France.


  1. 1.
    Global Initiative for Asthma (GINA) (2012) Pocket guide for asthma management and prevention.
  2. 2.
    Berend N, Salome CM, King GG (2008) Mechanisms of airway hyperresponsiveness in asthma. Respirology 13:624–631PubMedCrossRefGoogle Scholar
  3. 3.
    Bates JH, Rincon M, Irvin CG (2009) Animal models of asthma. Am J Physiol Lung Cell Mol Physiol 297(3):L401–L410PubMedCentralPubMedCrossRefGoogle Scholar
  4. 4.
    Epstein MM (2006) Are mouse models of allergic asthma useful for testing novel therapeutics? Exp Toxicol Pathol 57(Suppl 2):41–44PubMedCrossRefGoogle Scholar
  5. 5.
    Kumar RK, Herbert C, Foster PS (2008) The “classical” ovalbumin challenge model of asthma in mice. Curr Drug Targets 9(6):485–494PubMedCrossRefGoogle Scholar
  6. 6.
    Shinagawa K, Kojima M (2003) Mouse model of airway remodeling: strain differences. Am J Respir Crit Care Med 168(8):959–967PubMedCrossRefGoogle Scholar
  7. 7.
    Lukacs NW, John A, Berlin A, Bullard DC, Knibbs R, Stoolman LM (2002) E- and P-selectins are essential for the development of cockroach allergen-induced airway responses. J Immunol 169(4):2120–2125PubMedCrossRefGoogle Scholar
  8. 8.
    Ulrich K, Hincks JS, Walsh R et al (2008) Anti-inflammatory modulation of chronic airway inflammation in the murine house dust mite model. Pulm Pharmacol Ther 21:637–647PubMedCrossRefGoogle Scholar
  9. 9.
    Nials AT, Uddin S (2008) Mouse models of allergic asthma: acute and chronic allergen challenge. Dis Model Mech 1(4–5):213–220PubMedCentralPubMedCrossRefGoogle Scholar
  10. 10.
    McKee AS, Munks MW, MacLeod MK et al (2009) Alum induces innate immune responses through macrophage and mast cell sensors, but these sensors are not required for alum to act as an adjuvant for specific immunity. J Immunol 183:4403–4414PubMedCentralPubMedCrossRefGoogle Scholar
  11. 11.
    Zosky GR, Sly PD (2007) Animal models of asthma. Clin Exp Allergy 37(7):973–988PubMedCrossRefGoogle Scholar
  12. 12.
    Daubeuf F, Frossard N (2013) Acute asthma models to ovalbumin in the mouse. Curr Protocols Mouse Biol 3:31–37Google Scholar
  13. 13.
    Daubeuf F, Reber L, Frossard N (2013) Measurement of airway responsiveness on vigil and unrestrained mouse.
  14. 14.
    Daubeuf F, Reber L, Frossard N (2013) Measurement of airway responsiveness in the anesthetized mouse.
  15. 15.
    Daubeuf F, Frossard N (2012) Performing bronchoalveolar lavage in the mouse. Curr Protoc Mouse Biol 2:167–175Google Scholar
  16. 16.
    Delayre-Orthez C, Becker J, De Blay F, Frossard N, Pons F (2004) Dose-dependent effects of endotoxins on allergen sensitization and challenge in the mouse. Clin Exp Allergy 34:1789–1795Google Scholar
  17. 17.
    Daubeuf F, Hachet-Haas M, Gizzi P, Gasparik V, Bonnet D, Utard V, Hibert M, Frossard N, Galzi JL (2013) An antedrug of the CXCL12 neutraligand blocks experimental allergic asthma without systemic effect in mice. J Biol Chem 288(17):11865–11876Google Scholar
  18. 18.
    Gasparik V, Daubeuf F, Hachet-Haas M, Rohmer F, Gizzi P, Haiech J, Galzi JL, Hibert M, Bonnet D, Frossard N (2011) Prodrugs of a CXC chemokine ligand 12, CXCL12, neutraligand active in vivo in a new asthma model. ACS Med Chem Lett 3:10–14PubMedCentralPubMedCrossRefGoogle Scholar
  19. 19.
    Hachet-Haas M, Balabanian K, Rohmer F et al (2008) Small neutralizing molecules to inhibit actions of the chemokine CXCL12. J Biol Chem 283:23189–23199PubMedCrossRefGoogle Scholar
  20. 20.
    Andreasen CB (2003) Bronchoalveolar lavage. Vet Clin Small Anim 33:69–88CrossRefGoogle Scholar
  21. 21.
    Reber LL, Daubeuf F, Plantinga M, De Cauwer L, Gerlo S, Waelput W, Van Calenbergh S, Tavernier J, Haegeman G, Lambrecht BN, Frossard N, de Bosscher K (2012) A fully dissociated glucocorticoid receptor modulator reduces airway hyperresponsiveness and inflammation in a murine model of asthma. J Immunol 188:3478–3487PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Faculté de Pharmacie, Laboratoire d’Innovation Thérapeutique, LabEx MEDALIS, UMR7200CNRS-Université de StrasbourgIllkirchFrance

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