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Eosinophils pp 267-281 | Cite as

Antimicrobial Activity of Human Eosinophil Granule Proteins

  • Anu Chopra
  • Janendra K. BatraEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)

Abstract

Eosinophils secrete a number of proinflammatory mediators, like cytokines, chemokines, and granule proteins which are responsible for the initiation and sustenance of inflammatory response caused by them. The eosinophil granule proteins, ECP, EDN, MBP, and EPO possess antimicrobial activity against bacteria, helminths, protozoa, and viruses. In this chapter, we describe various assays used to detect and quantitate the antimicrobial activities of eosinophil granule proteins, particularly ECP and EDN. We have taken a model organism for each assay and described the method for antiviral, antihelminthic, antiprotozoan, and antibacterial activity of purified eosinophil granule proteins.

Key words

Eosinophil granule protein Antiviral Antibacterial Antihelminthic Antiprotozoan 

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Immunochemistry LaboratoryNational Institute of ImmunologyNew DelhiIndia
  2. 2.Centre for Molecular MedicineNational Institute of ImmunologyNew DelhiIndia

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