Eosinophils and Respiratory Virus Infection: A Dual-Standard Curve qRT-PCR-Based Method for Determining Virus Recovery from Mouse Lung Tissue
Several lines of investigation have indicated a role for eosinophilic leukocytes in limiting virus infectivity and promoting virion clearance. We have established a respiratory virus infection model with pneumonia virus of mice (PVM; family Paramyxoviridae), a natural mouse pathogen that replicates the more severe forms of human disease elicited by the phylogenetically related respiratory syncytial virus (RSV). In this chapter, we present a rapid and highly reproducible dual-standard curve qRT-PCR based method for quantitative detection of PVM replication in mouse lung tissue. We have used this assay to evaluate eosinophil-mediated antiviral host defense in mouse models of cytokine and antigen-driven eosinophilic inflammation.
Key wordsInflammation Leukocyte Eosinophil Pneumonia virus of mice Polymerase chain reaction
Support provided by NIAID Division of Intramural Research (AI000941 and AI000943) to HFR and Children’s Miracle Network of New York to JBD.
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