Advertisement

Eosinophils pp 251-255 | Cite as

Eosinophil Interactions: Antigen Presentation

  • Praveen AkuthotaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)

Abstract

Eosinophils have been demonstrated in a variety of experimental models to express MHC Class II and co-stimulatory molecules necessary to act as antigen presenting cells (APCs). They also have been experimentally observed to use these expressed proteins to function as APCs. This chapter reviews basic techniques to allow for in vitro and ex vivo experimentation of human and murine eosinophil APC function.

Key words

Eosinophils Antigen presentation Class II major histocompatibility antigens Granulocytes Humans 

Notes

Acknowledgments

This work was supported by a HOPE Pilot Grant from the American Partnership for Eosinophilic Disorders to P.A.

References

  1. 1.
    Lucey D, Nicholson-Weller A, Weller P (1989) Mature human eosinophils have the capacity to express HLA-DR. Proc Natl Acad Sci U S A 86:1348–1351PubMedCentralPubMedCrossRefGoogle Scholar
  2. 2.
    Wang H, Ghiran I, Matthaei K et al (2007) Airway eosinophils: allergic inflammation recruited professional antigen-presenting cells. J Immunol 179:7585–7592PubMedCentralPubMedCrossRefGoogle Scholar
  3. 3.
    Weller P, Rand T, Barrett T et al (1993) Accessory cell function of human eosinophils. HLA-DR-dependent, MHC-restricted antigen-presentation and IL-1 alpha expression. J Immunol 150:2554–2562PubMedGoogle Scholar
  4. 4.
    MacKenzie J, Mattes J, Dent L et al (2001) Eosinophils promote allergic disease of the lung by regulating CD4(+) Th2 lymphocyte function. J Immunol 167:3146–3155PubMedCrossRefGoogle Scholar
  5. 5.
    Mawhorter S, Pearlman E, Kazura J et al (1993) Class II major histocompatibility complex molecule expression on murine eosinophils activated in vivo by Brugia malayi. Infect Immun 61:5410–5412PubMedCentralPubMedGoogle Scholar
  6. 6.
    Hansel T, Braunstein J, Walker C et al (1991) Sputum eosinophils from asthmatics express ICAM-1 and HLA-DR. Clin Exp Immunol 86:271–277PubMedCentralPubMedCrossRefGoogle Scholar
  7. 7.
    Beninati W, Derdak S, Dixon P et al (1993) Pulmonary eosinophils express HLA-DR in chronic eosinophilic pneumonia. J Allergy Clin Immunol 92:442–449PubMedCrossRefGoogle Scholar
  8. 8.
    Patel A, Fuentebella J, Gernez Y et al (2010) Increased HLA-DR expression on tissue eosinophils in eosinophilic esophagitis. J Pediatr Gastroenterol Nutr 51:290–294PubMedGoogle Scholar
  9. 9.
    Celestin J, Rotschke O, Falk K et al (2001) IL-3 induces B7.2 (CD86) expression and costimulatory activity in human eosinophils. J Immunol 167:6097–6104PubMedCrossRefGoogle Scholar
  10. 10.
    Akuthota P, Wang H, Spencer L et al (2008) Immunoregulatory roles of eosinophils: a new look at a familiar cell. Clin Exp Allergy 38:1254–1263PubMedCentralPubMedCrossRefGoogle Scholar
  11. 11.
    Akuthota P, Spencer L, Melo R et al (2012) MHC Class II and CD9 in human eosinophils localize to detergent-resistant membrane microdomains. Am J Respir Cell Mol Biol 46:188–195PubMedCentralPubMedCrossRefGoogle Scholar
  12. 12.
    Shi H, Humbles A, Gerard C et al (2000) Lymph node trafficking and antigen presentation by endobronchial eosinophils. J Clin Invest 105:945–953PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonUSA
  2. 2.Division of Allergy and Inflammation, Department of Medicine, Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonUSA

Personalised recommendations