Eosinophils pp 231-249 | Cite as

Eosinophils Interaction with Mast Cells: The Allergic Effector Unit

  • Roopesh Singh Gangwar
  • Francesca Levi-SchafferEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)


Mast cells (MC) and eosinophils are the key effector cells of allergy (Minai-Fleminger and Levi-Schaffer, Inflamm Res 58:631–638, 2009). In general, allergic reactions have two phases, namely, an early phase and a late phase. MC and eosinophils abundantly coexist in the inflamed tissue in the late and chronic phases and cross talk in a bidirectional manner. This bidirectional interaction between MC and eosinophils is mediated by both physical cell–cell contacts through cell surface receptors such as CD48 , 2B4 and soluble mediators through various specific granular mediators, arachidonic acid metabolites, cytokines , and chemokines, collectively termed the “Allergic Effector Unit” (AEU) (Elishmereni et al., Allergy 66:376–385, 2011; Minai-Fleminger et al., Cell Tissue Res 341:405–415, 2010). These bidirectional interactions can be studied in vitro in a customized coculture system of MC and eosinophils derived from either mouse or human source.

Key words

Allergic Effector Unit Murine AEU Coculture Mast cell–eosinophil interactions BMMC BMEos 



The authors would like to thank to Laila Karra for her valuable comments and critical evaluation in writing this chapter. This work was supported by the Israel Science Foundation (grant 213/05), the MAARS EU 7th framework (grant no. HEALTH-F2-2011-261366), the Aimwell Charitable Trust (London, UK). F. Levi-Schaffer is affiliated with The Dr. Adolph and Klara Brettler Center for Research in Molecular Pharmacology and Therapeutics, School of Pharmacy and the Alex Grass Center for Drug Design and Synthesis of Novel Therapeutics, School of Pharmacy of The Hebrew University of Jerusalem.


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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Roopesh Singh Gangwar
    • 1
  • Francesca Levi-Schaffer
    • 2
    Email author
  1. 1.Department of Pharmacology and Experimental Therapeutics, Institute for Drug Research, School of Pharmacy, Faculty of MedicineThe Hebrew University of JerusalemJerusalemIsrael
  2. 2.Department of Pharmacology and Experimental Therapeutics, Institute for Drug Research, Faculty of MedicineThe Hebrew University of JerusalemJerusalemIsrael

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