Abstract
Eosinophils are granulocytes integral to allergic inflammation and parasitic responses and comprise 1–4 % of the circulating leukocytes in human beings under normal conditions. Isolation of human eosinophils allows for ex vivo and in vitro experimentation, providing a valuable tool for the study of allergic mechanisms. Here, we describe a technique for the isolation of human eosinophils by negative selection from whole blood obtained by venipuncture.
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Acknowledgments
This work was supported by a HOPE Pilot Grant from the American Partnership for Eosinophilic Disorders (to PA) and National Institutes of Health grants R01AI051645 and R37AI020241 (to PFW).
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Akuthota, P., Capron, K., Weller, P.F. (2014). Eosinophil Purification from Peripheral Blood. In: Walsh, G. (eds) Eosinophils. Methods in Molecular Biology, vol 1178. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1016-8_2
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DOI: https://doi.org/10.1007/978-1-4939-1016-8_2
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1015-1
Online ISBN: 978-1-4939-1016-8
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