Eosinophil Purification from Peripheral Blood
Eosinophils are granulocytes integral to allergic inflammation and parasitic responses and comprise 1–4 % of the circulating leukocytes in human beings under normal conditions. Isolation of human eosinophils allows for ex vivo and in vitro experimentation, providing a valuable tool for the study of allergic mechanisms. Here, we describe a technique for the isolation of human eosinophils by negative selection from whole blood obtained by venipuncture.
Key wordsEosinophils Cell separation/instrumentation Cell separation/methods Granulocytes Humans
This work was supported by a HOPE Pilot Grant from the American Partnership for Eosinophilic Disorders (to PA) and National Institutes of Health grants R01AI051645 and R37AI020241 (to PFW).