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Eosinophils pp 197-202 | Cite as

Clinical Measurement of Eosinophil Numbers in Eosinophilic Conjunctivitis

  • Osmo KariEmail author
  • K. Matti Saari
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)

Abstract

Cytological examination of conjunctival scrapings is a valuable technique in differentiating various types of conjunctivitis. Brush conjunctival cytology is easy to use, and it may show a rich cell sample also from the deeper conjunctival layers. It is atraumatic and suitable for tarsal conjunctival cytology. The Papanicolaou staining can be used for examination of epithelial cells and inflammatory cells. The semiquantitative counting method is rapid to use and gives some information about the severity and nature of the inflammation. Our modified method identifies the presence of eosinophils which are the hallmark both in allergic conjunctivitis and in non-allergic eosinophilic conjunctivitis (NAEC). NAEC is quite common affecting in most cases middle-aged or older people with the majority being women. NAEC is often connected with dry eye which in many cases can be seen in conjunctival cytology.

Key words

Conjunctivitis Counting cell numbers Dry eye Eosinophils Epithelium Goblet cells Inflammation Inflammatory cells 

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Helsinki University Central HospitalHelsinkiFinland
  2. 2.Department of OphthalmologyUniversity of TurkuTurkuFinland

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