Measurement of Eosinophil Kinetics in Healthy Volunteers
Radiolabelled leukocyte scans are widely used in nuclear medicine to locate sites of infection and inflammation. Radiolabelling of leukocyte subpopulations can also yield valuable information on cell trafficking and kinetics in vivo, but care must be taken to minimize inadvertent cell activation ex vivo. Here, we describe the use of autologous indium111-labelled eosinophils to measure eosinophil intravascular life-span and monitor their distribution and fate using gamma camera imaging in healthy non-atopic individuals.
Key wordsGranulocytes Eosinophils Radiolabelling Imaging Cell trafficking
This work was supported by Asthma UK [08/11]; the Medical Research Council [grant number MR/J00345X/1]; the Wellcome Trust [grant number 098351/Z/12/Z]; Biotechnology and Biological Sciences Research Council [grant number BB/H531100/1]; Papworth Hospital R&D; and Cambridge National Institute for Health Research Biomedical Research Centre.
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