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Eosinophils pp 165-176 | Cite as

Measurement of Eosinophil Kinetics in Healthy Volunteers

  • Neda FarahiEmail author
  • Chrystalla Loutsios
  • Rosalind P. Simmonds
  • Linsey Porter
  • Daniel Gillett
  • Sarah Heard
  • A. Michael Peters
  • Alison M. Condliffe
  • Edwin R. Chilvers
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)

Abstract

Radiolabelled leukocyte scans are widely used in nuclear medicine to locate sites of infection and inflammation. Radiolabelling of leukocyte subpopulations can also yield valuable information on cell trafficking and kinetics in vivo, but care must be taken to minimize inadvertent cell activation ex vivo. Here, we describe the use of autologous indium111-labelled eosinophils to measure eosinophil intravascular life-span and monitor their distribution and fate using gamma camera imaging in healthy non-atopic individuals.

Key words

Granulocytes Eosinophils Radiolabelling Imaging Cell trafficking 

Notes

Acknowledgements

This work was supported by Asthma UK [08/11]; the Medical Research Council [grant number MR/J00345X/1]; the Wellcome Trust [grant number 098351/Z/12/Z]; Biotechnology and Biological Sciences Research Council [grant number BB/H531100/1]; Papworth Hospital R&D; and Cambridge National Institute for Health Research Biomedical Research Centre.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Neda Farahi
    • 1
    Email author
  • Chrystalla Loutsios
    • 1
  • Rosalind P. Simmonds
    • 1
  • Linsey Porter
    • 1
  • Daniel Gillett
    • 2
  • Sarah Heard
    • 2
  • A. Michael Peters
    • 3
  • Alison M. Condliffe
    • 1
  • Edwin R. Chilvers
    • 1
  1. 1.Department of Medicine, Addenbrooke’s and Papworth HospitalsUniversity of Cambridge School of Clinical MedicineCambridgeUK
  2. 2.Nuclear MedicineCambridge University Hospitals National Health Service Foundation TrustCambridgeUK
  3. 3.Clinical Imaging Sciences CentreBrighton and Sussex Medical SchoolBrightonUK

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