Eosinophils pp 157-164 | Cite as

Human Eosinophil Transmigration

  • Stanislawa Bazan-SochaEmail author
  • Joanna Zuk
  • Bogdan Jakiela
  • Jacek Musial
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)


In this chapter we describe an optimized eosinophil transmigration assay. Transmigration of purified human peripheral blood eosinophils can be studied using special insert with membrane coated with extracellular matrix components or membrane covered with cells growing as a confluent monolayer, such as vascular endothelial cells of any origin or airway epithelial cells. In our opinion, eosinophil transmigration assay performed through monolayer of human microvascular endothelial cells of lung origin is a suitable tool to estimate the full migratory potential of eosinophils in studies on the pathology of asthma or other respiratory diseases, where eosinophils play important effector functions. This experimental system is easy to perform, simple for optimization, and comparable to in vivo processes occurring during eosinophil migration to the inflammatory sites in lungs.

Key words

Eosinophil Transmigration assay Endothelial cell monolayer Inflammation 



This work was supported by the State Committee for Scientific Research: registration number N N402 186835.


  1. 1.
    Bousquet J, Chanez A, Vignola J et al (1994) Eosinophil infiltration in asthma. Am J Respir Crit Care Med 150:33–38CrossRefGoogle Scholar
  2. 2.
    Bazan-Socha S, Bukiej A, Marcinkiewicz C et al (2005) Integrins in pulmonary inflammatory diseases. Curr Pharm Des 11:893–901PubMedCrossRefGoogle Scholar
  3. 3.
    Yamamoto H, Sedgwick JB, Vrtis RF et al (2000) The effect of transendothelial migration on eosinophil function. Am J Respir Cell Mol Biol 23:379–388PubMedCrossRefGoogle Scholar
  4. 4.
    Bazan-Socha S, Zuk J, Plutecka H et al (2012) Collagen receptors α1β1 and α2β1 integrins are involved in transmigration of peripheral blood eosinophils, but not mononuclear cells through human microvascular endothelial cells monolayer. J Physiol Pharmacol 63:373–379PubMedGoogle Scholar
  5. 5.
    Bazan-Socha S, Zuk J, Macinkiewicz C, et al. (2013) Collagen receptors: α1β1 and α2β1 integrins are involved in eosinophil transmigration through human microvascular endothelial cell lung monolayer. Abstract presented on EAACI conference, Milan, 22–26 June 2013. Abstract number: 523.Google Scholar

Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Stanislawa Bazan-Socha
    • 1
    Email author
  • Joanna Zuk
    • 1
  • Bogdan Jakiela
    • 1
  • Jacek Musial
    • 1
  1. 1.2nd Department of Internal MedicineJagiellonian University Medical CollegeKrakowPoland

Personalised recommendations