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Eosinophils pp 129-141 | Cite as

Human Eosinophil Adhesion and Receptor Expression

  • Colleen S. CurranEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)

Abstract

Eosinophils migrate from the bone marrow in response to cytokines and chemokines which induce the expression and activation of adhesion receptors. In understanding the recruitment of eosinophils, protocols to identify eosinophil adhesion and receptor expression have been identified. In this summary, the eosinophil peroxidase and fluorescent labeling assays are described as measurements of indirect and direct eosinophil adhesion, respectively. Additional protocols that identify eosinophil receptor expression via immunofluorescent microscopy and flow cytometry are also described.

Key words

Receptor Adhesion Peroxidase Flow cytometry Microscopy Eosinophil 

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Department of Cell and Regenerative BiologyUniversity of Wisconsin School of Medicine and Public HealthMadisonUSA

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