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Eosinophils pp 101-110 | Cite as

Eosinophil Chemotaxis

  • Gordon DentEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)

Abstract

Chemotaxis assays have a number of applications in the study of leukocyte biology and immune/inflammatory pathology. Multiwell “blind chamber”-type assays allow a large number of parallel measurements within a single assay. The development of fluorescence assays using microplate-based chemotaxis chambers has permitted a degree of automation to be applied to these assays. Here, a method is described for the quantitative measurement of eosinophil migration using a 96-well assay with numbers of cells that may realistically be obtained from blood samples.

Key words

Cell-based assays Chemotaxis Eosinophils Fluorescence Leukocytes Migration 

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  1. 1.Institute of Science & Technology in MedicineKeele UniversityKeele, StaffordshireUK

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