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Eosinophils pp 101–110Cite as

Eosinophil Chemotaxis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1178))

Abstract

Chemotaxis assays have a number of applications in the study of leukocyte biology and immune/inflammatory pathology. Multiwell “blind chamber”-type assays allow a large number of parallel measurements within a single assay. The development of fluorescence assays using microplate-based chemotaxis chambers has permitted a degree of automation to be applied to these assays. Here, a method is described for the quantitative measurement of eosinophil migration using a 96-well assay with numbers of cells that may realistically be obtained from blood samples.

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References

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Author information

Authors and Affiliations

  1. Institute of Science & Technology in Medicine, Keele University, Keele, Staffordshire, ST5 5BG, UK

    Gordon Dent

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  1. Gordon Dent
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Correspondence to Gordon Dent .

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Editors and Affiliations

  1. Division of Applied Medicine, University of Aberdeen Institute of Medical Sciences, Aberdeen, United Kingdom

    Garry M. Walsh

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© 2014 Springer Science+Business Media, New York

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Dent, G. (2014). Eosinophil Chemotaxis. In: Walsh, G. (eds) Eosinophils. Methods in Molecular Biology, vol 1178. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1016-8_10

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  • DOI: https://doi.org/10.1007/978-1-4939-1016-8_10

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1015-1

  • Online ISBN: 978-1-4939-1016-8

  • eBook Packages: Springer Protocols

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