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Virus-Induced Gene Silencing in Plant MAPK Research

  • Christian Hettenhausen
  • Ian T. Baldwin
  • Jianqiang WuEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1171)

Abstract

Virus-induced gene silencing (VIGS) technology has become more and more widely used in various plant species for rapid screening of gene functions. VIGS does not require time-consuming tissue culture steps that are needed for stable transformation in most plant species and it can be used for studying gene function even in plants that are very difficult to stably transform. Furthermore, VIGS technology provides high gene silencing efficiency (up to 95 %) and specificity. Here, we describe a VIGS protocol that can be used for studying the functions of MAPKs and other genes in a wild tobacco species, Nicotiana attenuata. This method is also suitable for other Nicotiana species and tomato with minor modifications.

Key words

Mitogen-activated protein kinase Nicotiana attenuata Virus-induced gene silencing Transient transformation 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Christian Hettenhausen
    • 1
  • Ian T. Baldwin
    • 2
  • Jianqiang Wu
    • 1
    Email author
  1. 1.Kunming Institute of Botany, Chinese Academy of SciencesHeilongtan, KunmingChina
  2. 2.Department of Molecular EcologyMax Planck Institute for Chemical EcologyJenaGermany

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