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Identification of Constitutively Active AtMPK6 Mutants Using a Functional Screen in Saccharomyces cerevisiae

  • Elodie Hudik
  • Souha Berriri
  • Heribert Hirt
  • Jean ColcombetEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1171)

Abstract

MAPK (Mitogen-Activated Protein Kinases) mutants which are active independently of phosphorylation by upstream MAPK Kinases (MAPKKs) help to clarify signal transduction processes through MAPK modules and provide a useful tool to understand MAPK roles in the cell. The identification of such mutations is tricky. In this chapter, we provide a detailed protocol for their screening, taking advantage of a functional expression assay in yeast.

Key words

MAP Kinase Functional genetic screen Constitutive activity Saccharomyces cerevisiae In vivo cloning Random mutagenesis 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Elodie Hudik
    • 1
  • Souha Berriri
    • 2
  • Heribert Hirt
    • 3
  • Jean Colcombet
    • 3
    Email author
  1. 1.Institut de Biologie des Plantes, UMR CNRS-8618Centre National De La Recherche ScientifiqueOrsay CedexFrance
  2. 2.Cell & Developmental Biology, Jones Innes CentreNorwich Research ParkNorwichUK
  3. 3.URGV Plant Genomics, UMR INRA-1165/CNRS-ERL8196Institut National De Recherche AgronomiqueEvry CedexFrance

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