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Cell Cycle Control pp 425–445Cite as

Detecting Senescence: Methods and Approaches

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1170))

Abstract

The detection of senescent cells has become an important area of research in the aging field. Due to the complexity of the senescence program and the lack of a unique signature for senescence, the detection of these cells remains problematic. This is especially true for in vivo detection in aged or diseased tissue samples. This chapter outlines approaches for the detection of senescent cells based upon methods established for mesenchymal cells in culture. A stepwise approach to the detection of senescent cells using multiple techniques is provided.

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Acknowledgements

This work was supported by grants 1RO1NS078283 to C.T. and AG039799 to C.S., the Commonwealth of Pennsylvania Universal Research Enhancement Grant (CT), the Drexel University College of Medicine Research Program Planning Grant (CT), and the Drexel Aging Initiative. T.N. is the recipient of a fellowship from the Drexel Aging Initiative. Research reported in this publication is also supported by the National Institute on Aging of the National Institutes of Health under Award Number F30AG043307 (EPC). The content of this chapter is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Authors and Affiliations

  1. Department of Pathology, Drexel University College of Medicine, 245 N. 15th Street, MS 435, Philadelphia, PA, 19102, USA

    Elizabeth P. Crowe, Timothy Nacarelli, Christian Sell & Claudio Torres

  2. Department of Pathology, University of Washington, Health Science Building D-514, 357470, Seattle, WA, USA

    Alessandro Bitto

  3. Department of Environmental Medicine, Lung Biology, and Disease Program, Pulmonary and Critical Care Division, University of Rochester Medical Center, Rochester, NY, USA

    Chad Lerner

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  1. Elizabeth P. Crowe
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  2. Timothy Nacarelli
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  3. Alessandro Bitto
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  4. Chad Lerner
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  5. Christian Sell
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  6. Claudio Torres
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Correspondence to Claudio Torres .

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Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA

    Eishi Noguchi

  2. Dept of Biochemistry & Molecular Biology, Drexel University College of Medicine, Philadelphia, USA

    Mariana C. Gadaleta

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Crowe, E.P., Nacarelli, T., Bitto, A., Lerner, C., Sell, C., Torres, C. (2014). Detecting Senescence: Methods and Approaches. In: Noguchi, E., Gadaleta, M. (eds) Cell Cycle Control. Methods in Molecular Biology, vol 1170. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0888-2_23

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  • DOI: https://doi.org/10.1007/978-1-4939-0888-2_23

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