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Cancer Cell Signaling pp 71–80Cite as

Use of Serum-Circulating miRNA Profiling for the Identification of Breast Cancer Biomarkers

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1165))

Abstract

MicroRNAs (miRNAs) are important regulatory molecules involved in disease pathogenesis. miRNAs are very stable in bodily fluids and can be detected in serum, plasma, saliva, and urine, among other fluids. Several studies have demonstrated the usefulness of serum miRNAs as potential biomarkers for detecting and monitoring cancer progression. Here, we describe in detail the experiment protocol we used to profile miRNA expression in the serum of breast cancer patients, including RNA extraction from serum, RT-qPCR quantification, and analysis of the deregulated miRNAs. Detection of circulating miRNAs may be a useful, noninvasive diagnostic tool for breast cancer.

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Acknowledgements

We would like to thank Perla K. Espino-Silva, Sandra K. Santuario-Facio, and Pablo Ruiz-Flores for the samples of breast cancer patients; Ismael Malagón-Santiago for statistical analysis; Alejandra Arreola-Triana for editorial review of this manuscript; and the Teachers’ Improvement Program (PROMEP) grants 103.5/07/2523 and 103.5/08/4285 for their financial support.

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Authors and Affiliations

  1. Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, UANL, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza, Nuevo León, C.P. 66451, México

    Fermín Mar-Aguilar, Cristina Rodríguez-Padilla & Diana Reséndez-Pérez

Authors
  1. Fermín Mar-Aguilar
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  2. Cristina Rodríguez-Padilla
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  3. Diana Reséndez-Pérez
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Corresponding author

Correspondence to Diana Reséndez-Pérez .

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Editors and Affiliations

  1. Universidad Nacional Autónoma de México, Mexico D.F., Mexico

    Martha Robles-Flores

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Mar-Aguilar, F., Rodríguez-Padilla, C., Reséndez-Pérez, D. (2014). Use of Serum-Circulating miRNA Profiling for the Identification of Breast Cancer Biomarkers. In: Robles-Flores, M. (eds) Cancer Cell Signaling. Methods in Molecular Biology, vol 1165. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0856-1_6

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  • DOI: https://doi.org/10.1007/978-1-4939-0856-1_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0855-4

  • Online ISBN: 978-1-4939-0856-1

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