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Use of Serum-Circulating miRNA Profiling for the Identification of Breast Cancer Biomarkers

  • Fermín Mar-Aguilar
  • Cristina Rodríguez-Padilla
  • Diana Reséndez-PérezEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1165)

Abstract

MicroRNAs (miRNAs) are important regulatory molecules involved in disease pathogenesis. miRNAs are very stable in bodily fluids and can be detected in serum, plasma, saliva, and urine, among other fluids. Several studies have demonstrated the usefulness of serum miRNAs as potential biomarkers for detecting and monitoring cancer progression. Here, we describe in detail the experiment protocol we used to profile miRNA expression in the serum of breast cancer patients, including RNA extraction from serum, RT-qPCR quantification, and analysis of the deregulated miRNAs. Detection of circulating miRNAs may be a useful, noninvasive diagnostic tool for breast cancer.

Key words

miRNAs Circulating microRNA Serum RT-qPCR Blood Biomarker Breast cancer 

Notes

Acknowledgements

We would like to thank Perla K. Espino-Silva, Sandra K. Santuario-Facio, and Pablo Ruiz-Flores for the samples of breast cancer patients; Ismael Malagón-Santiago for statistical analysis; Alejandra Arreola-Triana for editorial review of this manuscript; and the Teachers’ Improvement Program (PROMEP) grants 103.5/07/2523 and 103.5/08/4285 for their financial support.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Fermín Mar-Aguilar
    • 1
  • Cristina Rodríguez-Padilla
    • 1
  • Diana Reséndez-Pérez
    • 1
    Email author
  1. 1.Laboratorio de Inmunología y Virología, Facultad de Ciencias BiológicasUniversidad Autónoma de Nuevo León, UANL, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los GarzaNuevo LeónMéxico

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