MicroRNAs (miRNAs) are important regulatory molecules involved in disease pathogenesis. miRNAs are very stable in bodily fluids and can be detected in serum, plasma, saliva, and urine, among other fluids. Several studies have demonstrated the usefulness of serum miRNAs as potential biomarkers for detecting and monitoring cancer progression. Here, we describe in detail the experiment protocol we used to profile miRNA expression in the serum of breast cancer patients, including RNA extraction from serum, RT-qPCR quantification, and analysis of the deregulated miRNAs. Detection of circulating miRNAs may be a useful, noninvasive diagnostic tool for breast cancer.
miRNAs Circulating microRNA Serum RT-qPCR Blood Biomarker Breast cancer
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We would like to thank Perla K. Espino-Silva, Sandra K. Santuario-Facio, and Pablo Ruiz-Flores for the samples of breast cancer patients; Ismael Malagón-Santiago for statistical analysis; Alejandra Arreola-Triana for editorial review of this manuscript; and the Teachers’ Improvement Program (PROMEP) grants 103.5/07/2523 and 103.5/08/4285 for their financial support.
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