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Gene Disruption Using Zinc Finger Nuclease Technology

  • Sara Granja
  • Ibtissam Marchiq
  • Fátima BaltazarEmail author
  • Jacques Pouysségur
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1165)

Abstract

Zinc finger nucleases are reagents that induce DNA double-strand breaks at specific sites that can be repaired by nonhomologous end joining, inducing alterations in the genome. This strategy has enabled highly efficient gene disruption in numerous cell types and model organisms opening a door for new therapeutic applications. Here, we describe the disruption of CD147/basigin by this technique in a human cancer cell line.

Key words

DNA double-strand breaks Gene targeting Knockout Zinc finger nuclease 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Sara Granja
    • 1
    • 2
  • Ibtissam Marchiq
    • 3
  • Fátima Baltazar
    • 1
    • 2
    Email author
  • Jacques Pouysségur
    • 3
  1. 1.Surgical Sciences DomainLife and Health Sciences Research Institute (ICVS), School of Health Sciences, University of MinhoBragaPortugal
  2. 2.ICVS/3B’s-PT Government Associate LaboratoryBraga/GuimarãesPortugal
  3. 3.Institute for Research on Cancer and Aging, University of Nice, CNRS, InsermNiceFrance

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