A Flow Cytometry-Based Assay for the Evaluation of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) in Cancer Cells

  • Nohemí Salinas-Jazmín
  • Emiliano Hisaki-Itaya
  • Marco A. Velasco-VelázquezEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1165)


The development of new therapeutic monoclonal antibodies (mAbs) for cancer therapy will rise in the following years. The evaluation of biological activity of mAbs is required during drug development and during drug production as quality control test. Antibody-dependent cell-mediated cytotoxicity (ADCC) is a desirable activity of anticancer mAbs. Here, we describe a flow cytometry-based method to quantify ADCC that combines the staining of cancer cells, effector cells, and dead cells, with specific dyes. This method is inexpensive, has low background, and avoids the use of radioisotopes.

Key words

ADCC Flow cytometry Trastuzumab 7-AAD Biosimilar 



We thank Dr. Frank Robledo-Avila and Dra. Mayra Pérez-Tapia for the critical review of this manuscript. Assays presented in this work were performed at “Unidad de Desarrollo e Investigación en Bioprocesos (UDIBI), Instituto Politécnico Nacional.” The work is supported by a grant from “Instituto Científico Pfizer, México” and grants IN219613 (PAPIIT-UNAM) and 225313 (Conacyt) to MAV-V.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Nohemí Salinas-Jazmín
    • 1
  • Emiliano Hisaki-Itaya
    • 1
  • Marco A. Velasco-Velázquez
    • 2
    Email author
  1. 1.Unidad de Desarrollo e Investigación en Bioprocesos (UDIBI)Escuela Nacional de Ciencias BiológicasMéxicoMéxico
  2. 2.Departamento de Farmacología, Facultad de MedicinaUNAMMéxicoMéxico

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