Zebrafish-Based Systems Pharmacology of Cancer Metastasis

  • Yasuhito Shimada
  • Yuhei Nishimura
  • Toshio TanakaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1165)


Because of their small size, high fecundity, and commonality to human genetics and genomics, phenotype-based animal testing using zebrafish (Danio rerio) has emerged as a powerful tool for identifying disease mechanisms, drug target molecules and small bioactive compounds over the last decade. Importantly, the immaturity of the zebrafish larvae immune system compared with that of mammals facilitates the implantation of human tumors representing aggressive cancer progression with metastasis. In the current chapter, we describe the methods for human cancer cell xenotransplantation into zebrafish, phenotypic image analysis, and transcriptome analysis using deep sequencing.

Key words

Cancer transplantation Next-generation sequencer RNA-seq Transcriptome analysis Bioinformatics Pathway analysis 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Yasuhito Shimada
    • 1
    • 2
    • 3
    • 4
    • 5
  • Yuhei Nishimura
    • 1
    • 2
    • 3
    • 4
    • 5
  • Toshio Tanaka
    • 1
    • 2
    • 3
    • 4
    • 5
    Email author
  1. 1.Department of Molecular and Cellular Pharmacology, Pharmacogenomics and PharmacoinformaticsMie University Graduate School of MedicineTsuJapan
  2. 2.Department of Systems PharmacologyMie University Graduate School of MedicineTsuJapan
  3. 3.Mie University Medical Zebrafish Research CenterTsuJapan
  4. 4.Department of BioinformaticsMie University Life Science Research CenterTsuJapan
  5. 5.Department of Omics MedicineMie University Industrial Technology Innovation InstituteTsuJapan

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