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Use of the Tumor Repressor DEDD as a Prognostic Marker of Cancer Metastasis

  • Qi Lv
  • Fang Hua
  • Zhuo-Wei HuEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1165)

Abstract

DEDD, a member of a family of death effector domain-containing proteins, plays crucial roles in mediating apoptosis, regulating cell cycle, and inhibiting cell mitosis. Our recent work demonstrates that DEDD is a novel tumor repressor, which impedes metastasis by reversing the epithelial–mesenchymal transition (EMT) process in breast and colon cancers. DEDD expression therefore may represent a prognostic marker and potential therapeutic target for the prevention and treatment of cancer metastasis. To reveal the anti-metastatic roles of DEDD in these cancer cells, a number of experiments, including immunohistochemistry, the establishment of stably overexpressing or silencing cancer cells, chemoinvasion assay, soft agar assay, protein degradation, and protein–protein interaction were used in our in vitro and in vivo studies. This chapter focuses on the details of these experiments to provide references for the researchers to investigate the function of a gene in the regulation of tumor metastasis.

Key words

Anchorage independent growth DEDD Immunohistochemistry Invasion assay Metastasis Protein degradation Protein interaction 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Molecular Immunology and Cancer Pharmacology Group, State Key Laboratory of Bioactive Substance and Function of Natural MedicinesInstitute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical CollegeBeijingPeople’s Republic of China

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