Abstract
Infrared thermal analysis is an invaluable technique to study the plant freezing process. In the differential mode infrared thermal analysis allows to localize ice nucleation and ice propagation in whole plants or plant samples at the tissue level. Ice barriers can be visualized, and supercooling of cells, tissues, and organs can be monitored. Places where ice masses are accommodated in the apoplast can be identified. Here, we describe an experimental setting developed in the laboratory in Innsbruck, give detailed information on the practical procedure and preconditions, and give additionally an idea of the problems that can be encountered and how they by special precautions may be overcome.
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References
Wisniewski ME, Gusta LV, Fuller MP, Karlson D (2009) Ice nucleation, propagation and deep supercooling: the lost tribes of freezing studies. In: Gusta LV, Wisniewski ME, Tanino KK (eds) Plant cold hardiness: from the laboratory to the field. CAB International, Cambridge, pp 1–11
Gusta LV, Wisniewski ME, Trischuk RG (2009) Patterns of freezing in plants: the influence of species, environment and experimental procedures. In: Gusta LV, Wisniewski ME, Tanino KK (eds) Plant cold hardiness: from the laboratory to the field. CAB International, Cambridge, pp 214–223
Hacker J, Neuner G (2007) Ice propagation in plants visualized at the tissue level by infrared differential thermal analysis (IDTA). Tree Physiol 27:1661–1670
Hacker J, Neuner G (2008) Ice propagation in dehardened alpine plant species studied by infrared differential thermal analysis (IDTA). Arc Antarc Alp Res 40:660–670
Hacker J, Spindelböck J, Neuner G (2008) Mesophyll freezing and effects of freeze dehydration visualized by simultaneous measurement of IDTA and differential imaging chlorophyll fluorescence. Plant Cell Environ 31:1725–1733
Franks F (1985) Biophysics and biochemistry at low temperatures. Cambridge University Press, Cambridge
Chen S-H, Mallamace F, Mou C-Y, Broco M, Corsavo C, Faraone A, Liu L (2006) The violation of the Stokes-Einstein relation in supercooled water. Proc Natl Acad Sci U S A 103: 12974–12978
Sakai A, Larcher W (1987) Frost survival of plants. Responses and adaptation to freezing stress. In: Billings WD, Golley F, Lange OL, Olson JS, Remmert H (eds) Ecological studies, vol 62. Springer, New York
Burke MJ, Gusta LV, Quamme HA, Weiser CJ, Li PH (1976) Freezing and injury in plants. Annu Rev Plant Physiol Plant Mol Biol 27: 507–528
Wisniewski M, Lindow SE, Ashworth EN (1997) Observations of ice nucleation and propagation in plants using infrared video thermography. Plant Physiol 113:327–334
Lutze JL, Roden JS, Holly CJ, Wolfe J, Egerton JJG, Ball MC (1998) Elevated atmospheric [CO2] promotes frost damage in evergreen tree seedlings. Plant Cell Environ 21:631–635
Ball MC, Wolfe J, Canny M, Hofmann M, Nicotra AB, Hughes D (2002) Space and time dependence of temperature and freezing in evergreen leaves. Funct Plant Biol 29: 1259–1272
Sekozawa Y, Sugaya S, Gemma H (2004) Observations of ice nucleation and propagation in flowers of Japanese Pear (Pyrus pyrifolia Nakai) using infrared video thermography. J Jpn Soc Hortic Sci 73:1–6
Neuner G, Xu BC, Hacker J (2010) Velocity and pattern of ice propagation and deep supercooling in woody stems of Castanea sativa, Morus nigra and Quercus robur measured by IDTA. Tree Physiol 30:1037–1045
Hacker J, Ladinig U, Wagner J, Neuner G (2011) Inflorescences of alpine cushion plants freeze autonomously and may survive subzero temperatures by supercooling. Plant Sci 180: 149–156
Pramsohler M, Hacker J, Neuner G (2012) Freezing pattern and frost killing temperature of apple (Malus domestica) wood under controlled conditions and in nature. Tree Physiol 32:819–828
Neuner G, Buchner O (1999) Assessment of foliar frost damage: a comparison of in vivo chlorophyll fluorescence with other viability tests. J Appl Bot 73:50–54
Pearce RS (2001) Plant freezing and damage. Ann Bot 87:417–424
Neuner G, Hacker J (2012) Ice formation and propagation in alpine plants. In: Lütz C (ed) Plants in alpine regions: cell physiology of adaptation and survival strategies. Springer, Wien, pp 163–174
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Neuner, G., Kuprian, E. (2014). Infrared Thermal Analysis of Plant Freezing Processes. In: Hincha, D., Zuther, E. (eds) Plant Cold Acclimation. Methods in Molecular Biology, vol 1166. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0844-8_9
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DOI: https://doi.org/10.1007/978-1-4939-0844-8_9
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