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Conducting Field Trials for Frost Tolerance Breeding in Cereals

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Plant Cold Acclimation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1166))

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Abstract

Cereal species can be damaged by frost either during winter or at flowering stage. Frost tolerance per se is only a part of the mechanisms that allow the plants to survive during winter; winterhardiness also considers other biotic or physical stresses that challenge the plants during the winter season limiting their survival rate. While frost tolerance can also be tested in controlled environments, winterhardiness can be determined only with field evaluations. Post-heading frost damage occurs from radiation frost events in spring during the reproductive stages. A reliable evaluation of winterhardiness or of post-heading frost damage should be carried out with field trials replicated across years and locations to overcome the irregular occurrence of natural conditions which satisfactorily differentiate genotypes. The evaluation of post-heading frost damage requires a specific attention to plant phenology. The extent of frost damage is usually determined with a visual score at the end of the winter.

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References

  1. Fowler DB, Carles RJ (1979) Growth, development, and cold tolerance of fall-acclimated cereal grains. Crop Sci 19:915–922

    Article  Google Scholar 

  2. Trevaskis B, Hemming MN, Dennis ES, Peacock WJ (2007) The molecular basis of vernalization-induced flowering in cereals. Trends Plant Sci 12:352–357

    Article  CAS  PubMed  Google Scholar 

  3. von Zitzewitz J, Szucs P, Dubcovsky J, Yan LL, Francia E, Pecchioni N, Casas A, Chen THH, Hayes PM, Skinner JS (2005) Molecular and structural characterization of barley vernalization genes. Plant Mol Biol 59:449–467

    Article  CAS  Google Scholar 

  4. Yan L, Loukoianov A, Tranquilli G, Helguera M, Fahima T, Dubcovsky J (2003) Positional cloning of the wheat vernalization gene VRN1. Proc Natl Acad Sci U S A 100:6263–6268

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  5. Rizza F, Pagani D, Gut M, Prasil IT, Lago C, Tondelli A, OrrĂ¹ L, Mazzucotelli E, Francia E, Badeck FW, Crosatti C, Terzi V, Cattivelli L, Stanca AM (2011) Diversity in the response to low temperature in a set of representative barley genotypes cultivated in Europe. Crop Sci 51:2759–2779

    Article  CAS  Google Scholar 

  6. von Zitzewitz J, Cuesta-Marcos A, Condon F, Castro AJ, Chao S, Corey A, Filichkin T, Fisk SP, Gutierrez L, Haggard K, Karsai I, Muehlbauer GJ, Smith KP, Veisz O, Hayes PM (2011) The genetics of winter-hardiness in barley: perspectives from genome-wide association mapping. Plant Genome 4:76–91

    Article  Google Scholar 

  7. VĂ¡gĂºjfalvi A, Galiba G, Cattivelli L, Dubcovsky J (2003) The cold regulated transcriptional activator Cbf3 is linked to the frost-tolerance gene Fr-A2 on wheat chromosome 5A. Mol Genet Genomics 269:60–67

    PubMed  Google Scholar 

  8. VĂ¡gĂºjfalvi A, Aprile A, Miller A, Dubcovsky J, Delogu G, Galiba G, Cattivelli L (2005) The expression of several Cbf genes at the Fr-A2 locus is linked to frost resistance in wheat. Mol Genet Genomics 274:506–514

    Article  PubMed  Google Scholar 

  9. Francia E, Rizza F, Cattivelli L, Stanca AM, Galiba G, TĂ³th B, Hayes PM, Skinner JS, Pecchioni N (2004) Two loci on chromosome 5H determine low temperature tolerance in the new ‘winter’ Ă— ‘spring’ (‘Nure’ Ă— ‘Tremois’) barley map. Theor Appl Genet 108:670–680

    Article  CAS  PubMed  Google Scholar 

  10. Tondelli A, Barabaschi D, Francia E, Pecchioni N (2011) Inside the CBF locus in Gramineae. Plant Sci 180:39–45

    Article  CAS  PubMed  Google Scholar 

  11. Cattivelli L (2011) More cold tolerant plants in a warmer world. Plant Sci 180:1–2

    Article  CAS  PubMed  Google Scholar 

  12. Reinheimer JL, Barr AR, Eglinton JK (2004) QTL mapping of chromosomal regions conferring reproductive frost tolerance in barley (Hordeum vulgare L.). Theor Appl Genet 109:1267–1274

    Article  CAS  PubMed  Google Scholar 

  13. Fuller MP, Fuller AM, Kaniouras S, Christopher J, Fredericks T (2007) The freezing characteristics of wheat at ear emergence. Eur J Agron 26:435–441

    Article  Google Scholar 

  14. Crosatti C, Pagani D, Cattivelli L, Stanca AM, Rizza F (2008) Effects of the growth stage and hardening conditions on the association between frost resistance and the expression of the cold induced protein COR14b in barley. Environ Exp Bot 62:93–100

    Article  CAS  Google Scholar 

  15. Rizza F, Crosatti C, Stanca AM, Cattivelli L (1994) Studies for assessing the influence of hardening on cold tolerance of barley genotypes. Euphytica 75:131–138

    Article  Google Scholar 

  16. Beniston M (2005) Warm winter spells in the Swiss Alps: strong heat waves in a cold season? A study focusing on climate observations at the Saentis high mountain site. Geophys Res Lett 32, L01812

    Article  Google Scholar 

  17. Shabbar A, Bonasal B (2003) An assessment of changes in winter cold and warm spells over Canada. Nat Hazards 29:173–188

    Article  Google Scholar 

  18. Achard P, Gong F, Cheminant S, Alioua M, Hedden P, Genschik P (2008) The cold-inducible CBF1 factor-dependent signaling pathway modulates the accumulation of the growth-repressing DELLA proteins via its effect on gibberellin metabolism. Plant Cell 20:2117–2129

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. Gaudet DA (1994) Progress towards understanding interaction between cold hardiness and snow mould resistance and development of resistant cultivars. Can J Plant Pathol 16:241–246

    Article  Google Scholar 

  20. Andrews CJ, Pomeroy MK (1979) Toxicity of anaerobic metabolites accumulating in winter wheat seedlings during ice encasement. Plant Physiol 64:120–125

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. Gaudet DA, Wang Y, Frick M, Puchalski B, Penniket C, Ouellet T, Robert L, Singh J, Laroche A (2011) Low temperature induced defence gene expression in winter wheat in relation to resistance to snow moulds and other wheat diseases. Plant Sci 180:99–110

    Article  CAS  PubMed  Google Scholar 

  22. Frederiks TM, Christopher JT, Harvey GL, Sutherland MW, Borrell AK (2012) Current and emerging screening methods to identify posthead-emergence frost adaptation in wheat and barley. J Exp Bot 63:5405–5416

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Luigi Cattivelli .

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Cattivelli, L. (2014). Conducting Field Trials for Frost Tolerance Breeding in Cereals. In: Hincha, D., Zuther, E. (eds) Plant Cold Acclimation. Methods in Molecular Biology, vol 1166. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0844-8_4

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  • DOI: https://doi.org/10.1007/978-1-4939-0844-8_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0843-1

  • Online ISBN: 978-1-4939-0844-8

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