Abstract
Histological analysis of frozen and thawed plants has been conducted for many years but the observation of individual sections provides only a 2-dimensional representation of a 3-dimensional phenomenon. Most techniques for viewing internal plant structure in three dimensions are either low in resolution or the instrument cannot penetrate deep enough into the tissue to visualize the whole plant. Techniques with higher resolution are expensive and equipment often requires time-consuming training. We present a relatively simple and less-expensive technique using pixel-based (JPEG) images of histological sections of an Arabidopsis thaliana plant and commercially available software to generate 3D reconstructions of internal structures. The technique has proven to work just as effectively for images from medical histology.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Peyrin F (2009) Investigation of bone with synchrotron radiation imaging: from micro to nano. Osteoporos Int 20:1057–1063
Ferrando M, Spiess WEL (2000) Review: confocal scanning laser microscopy. A powerful tool in food science. Food Sci Tech Int 6:267–284
Livingston DP III, Tuong TD, Gadi SRV, Haigler CH, Gelman RS, Cullen JM (2010) 3D reconstructions with pixel-based images are made possible by digitally clearing plant and animal tissue. J Microsc 240:122–129
Livingston DP III, Tuong TD, Haigler CH, Avci U, Tallury SP (2009) Rapid microwave tissue processing of winter cereals for histology allows identification of separate zones of freezing injury in the crown. Crop Sci 49:1837–1842
Livingston DP III, Herman EM, Premakumar R, Tallury SP (2007) Using Arabidopsis thaliana as a model to study subzero acclimation in small grains. Cryobiology 54:154–163
Tanino KK, McKersie BD (1985) Injury within the crown of winter wheat seedlings after freezing and icing stress. Can J Bot 63:432–435
Olien CR, Marchetti BL (1976) Recovery of hardened barley from winter injuries. Crop Sci 16:201–204
Livingston DP III, Henson CA, Tuong TD, Wise ML, Tallury SP, Stanley H, Duke SH (2013) Histological analysis and 3D reconstruction of winter cereal crowns recovering from freezing: a unique response in oat (Avena sativa L.). PLoS One 8:e53468. doi:10.1371/journal.pone. 0053468
Johansen DA (1940) Stains. In: Plant microtechnique. McGraw-Hill, New York, p 49–64
Beckman CH (2000) Phenolic-storing cells: keys to programmed cell death and periderm formation in wilt disease resistance in general defense responses in plants? Physiol Mol Plant Pathol 57:101–110
Marshall D (1988) A relationship between ice-nucleation-active bacteria, freeze damage, and genotype in oats. Phytopathology 78:952–957
Olien CR, Smith MN (1981) Extension of localized freeze injury in barley by acute post-thaw bacterial disease. Cryobiology 18:404–409
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
1 Supplementary Materials
Below is the link to the electronic supplementary material.
(MP4 10,792 kb)
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Livingston, D.P., Tuong, T.D. (2014). Three-Dimensional Reconstruction of Frozen and Thawed Plant Tissues from Microscopic Images. In: Hincha, D., Zuther, E. (eds) Plant Cold Acclimation. Methods in Molecular Biology, vol 1166. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0844-8_11
Download citation
DOI: https://doi.org/10.1007/978-1-4939-0844-8_11
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0843-1
Online ISBN: 978-1-4939-0844-8
eBook Packages: Springer Protocols