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Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method

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Yeast Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1163))

Abstract

Transformation is essential to many molecular and genetic investigations in the yeast Saccharomyces cerevisiae. Yeast transformation protocols utilizing the LiAc/ssDNA/PEG method are presented. Protocols for various applications are listed including a method for transformation in 96-well microtiter plate format and another for the production of frozen competent yeast cells that can be used at a moment’s notice.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Avenue, Winnipeg, MB, Canada, R3E 0W3

    R. Daniel Gietz

Authors
  1. R. Daniel Gietz
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Corresponding author

Correspondence to R. Daniel Gietz .

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Editors and Affiliations

  1. Capital Normal University College of Life Sciences, Beijing, China

    Wei Xiao

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Gietz, R.D. (2014). Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method. In: Xiao, W. (eds) Yeast Protocols. Methods in Molecular Biology, vol 1163. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0799-1_4

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  • DOI: https://doi.org/10.1007/978-1-4939-0799-1_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0798-4

  • Online ISBN: 978-1-4939-0799-1

  • eBook Packages: Springer Protocols

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