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Ligand Engineering Using Yeast Surface Display

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Yeast Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1163))

Abstract

The genotype–phenotype linkage provided by display technologies enables efficient synthesis, analysis, and selection of combinatorial protein libraries. This approach tremendously expands the protein sequence space that can be efficiently evaluated for a selectable function. It thereby provides a key element in identification and directed evolution of novel or improved protein function. Here, yeast surface display is described in the context of selection for binding function. Yeast culture and multiple approaches to magnetic- and fluorescence-based protein selection are presented in detail.

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Acknowledgement

The preparation of this manuscript was supported by the University of Minnesota.

Author information

Authors and Affiliations

  1. Department of Chemical Engineering and Materials Science, University of Minnesota, Twin Cities, 356 Amundson Hall, 421 Washington Avenue SE, Minneapolis, MN, 55455, USA

    Benjamin J. Hackel

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  1. Benjamin J. Hackel
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Correspondence to Benjamin J. Hackel .

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Editors and Affiliations

  1. Capital Normal University College of Life Sciences, Beijing, China

    Wei Xiao

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Hackel, B.J. (2014). Ligand Engineering Using Yeast Surface Display. In: Xiao, W. (eds) Yeast Protocols. Methods in Molecular Biology, vol 1163. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0799-1_20

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  • DOI: https://doi.org/10.1007/978-1-4939-0799-1_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0798-4

  • Online ISBN: 978-1-4939-0799-1

  • eBook Packages: Springer Protocols

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