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Axon Growth and Regeneration pp 167–175Cite as

In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regeneration

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1162))

Abstract

Electroporation has been a widely used tool to introduce DNA plasmids or RNA oligos into cultured cells and recently in vivo into chick or mouse embryos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain- and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.

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References

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Acknowledgments

This work was supported by grants to F.Q.Z. from NIH (R01NS064288) and the Craig H. Neilsen Foundation.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, The Johns Hopkins University, Baltimore, MD, USA

    Saijilafu, Bo-Yin Zhang & Feng-Quan Zhou

  2. The Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University, Room 291, The John G. Rangos. Sr. Building, 855 North Wolfe Street, Baltimore, MD, 21205, USA

    Feng-Quan Zhou

Authors
  1. Saijilafu
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  2. Bo-Yin Zhang
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  3. Feng-Quan Zhou
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Corresponding author

Correspondence to Feng-Quan Zhou .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York, USA

    Andrew J. Murray

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Saijilafu, Zhang, BY., Zhou, FQ. (2014). In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regeneration. In: Murray, A. (eds) Axon Growth and Regeneration. Methods in Molecular Biology, vol 1162. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0777-9_14

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  • DOI: https://doi.org/10.1007/978-1-4939-0777-9_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0776-2

  • Online ISBN: 978-1-4939-0777-9

  • eBook Packages: Springer Protocols

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