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Simultaneous Multi-Wavelength Optical Imaging of Neuronal and Hemodynamic Activity

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Neurovascular Coupling Methods

Part of the book series: Neuromethods ((NM,volume 88))

Abstract

Functional brain imaging techniques, such as functional magnetic resonance imaging, infer changes in underlying neural activity from perfusion-related signals and can be sampled from large areas of cortex to examine hemodynamic network activity. However, measurement of the underlying neuronal activity in a correspondingly widespread network is not available for comparison. In order to accurately understand the origins of these hemodynamic signals and the mechanisms of neurovascular coupling, it is critical to be able to measure widespread neuronal activity simultaneously and homotopically with the vascular signal. For this reason, we designed a simultaneous multi-wavelength optical imaging system that can provide high-resolution spatiotemporal information of neuronal and hemodynamic activities, and their inter-relationship. We have demonstrated that this technique is an effective method with which to study neurovascular coupling during spontaneous brain activity, and pathological conditions such as epilepsy.

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Author information

Authors and Affiliations

  1. Department of Neurological Surgery, Brain and Mind Research Institute, Weill Cornell Medical College, 1300 York Avenue, New York, NY, 10065, USA

    Hongtao Ma Ph.D. & Mingrui Zhao Ph.D.

  2. Department of Psychology, The University of Sheffield, Sheffield, UK

    Samuel Harris

  3. Department of Neurological Surgery, Weill Cornell Medical College, New York, NY, USA

    Samuel Harris

  4. Department of Neurological Surgery, Brain and Mind Research Institute, Weill Cornell Medical College, New York Presbyterian Hospital, New York, NY, USA

    Theodore H. Schwartz

Authors
  1. Hongtao Ma Ph.D.
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  2. Mingrui Zhao Ph.D.
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  3. Samuel Harris
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  4. Theodore H. Schwartz
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Corresponding author

Correspondence to Hongtao Ma Ph.D. .

Editor information

Editors and Affiliations

  1. Weill Cornell Medical College, New York, New York, USA

    Mingrui Zhao

  2. Weill Cornell Medical College, New York, New York, USA

    Hongtao Ma

  3. Weill Cornell Medical College, New York, New York, USA

    Theodore H. Schwartz

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Ma, H., Zhao, M., Harris, S., Schwartz, T.H. (2014). Simultaneous Multi-Wavelength Optical Imaging of Neuronal and Hemodynamic Activity. In: Zhao, M., Ma, H., Schwartz, T. (eds) Neurovascular Coupling Methods. Neuromethods, vol 88. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0724-3_12

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  • DOI: https://doi.org/10.1007/978-1-4939-0724-3_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0723-6

  • Online ISBN: 978-1-4939-0724-3

  • eBook Packages: Springer Protocols

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