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Multilocus Sequence Typing (MLST) of Listeria monocytogenes

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Listeria monocytogenes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1157))

Abstract

Nucleotide sequence-based methods focusing on the single nucleotide polymorphisms (SNPs) of Listeria monocytogenes housekeeping genes facilitate the rapid and interlaboratory comparison on open accessible databases, such as the multilocus sequence typing (MLST) databases that are available. MLST has advantages over other methods as it can reconstruct ancestral and evolutionary linkage between L. monocytogenes isolates. MLST detects all genetic variations within the amplified housekeeping gene that accumulate slowly. This chapter describes how to undertake MLST.

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Acknowledgements

The authors wish to acknowledge the Institute Pasteur for providing the MLST database of L. monocytogenes in the Genotyping of Pathogens and Public Health Platform.

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Authors and Affiliations

  1. Department of Veterinary Public Health and Food Science, Institute of Milk Hygiene, Milk Technology and Food Science, University of Veterinary Medicine, Veterinärplatz 1, 1210, Vienna, Austria

    Beatrix Stessl, Irene Rückerl & Martin Wagner

  2. Department of Nutritional Sciences, Faculty of Life Sciences, University of Vienna, Althanstraße 14, 1090, Vienna, Austria

    Irene Rückerl

Authors
  1. Beatrix Stessl
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  2. Irene Rückerl
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  3. Martin Wagner
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Corresponding author

Correspondence to Beatrix Stessl .

Editor information

Editors and Affiliations

  1. Teagasc Food Research Centre, Fermoy, Cork, Ireland

    Kieran Jordan

  2. Food Microbiology and Safety Group, Animal, Food and Health Sciences, CSIRO, Werribee, Victoria, Australia

    Edward M. Fox

  3. University of Veterinary Medicine, Vienna, Austria

    Martin Wagner

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Stessl, B., Rückerl, I., Wagner, M. (2014). Multilocus Sequence Typing (MLST) of Listeria monocytogenes . In: Jordan, K., Fox, E., Wagner, M. (eds) Listeria monocytogenes. Methods in Molecular Biology, vol 1157. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0703-8_6

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  • DOI: https://doi.org/10.1007/978-1-4939-0703-8_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0702-1

  • Online ISBN: 978-1-4939-0703-8

  • eBook Packages: Springer Protocols

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