Abstract
Reverse transcription combined with the polymerase chain reaction (RT-PCR) is a viable method widely used to quantify gene expression. There are two ways to quantify gene expression by real-time PCR: relative quantification and absolute quantification. Relative quantification relates the PCR signal of the target gene to a control gene, normally a housekeeping gene. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Here we describe both methods from RNA extraction to its quantification by real-time PCR.
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References
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Mazza, R., Mazzette, R. (2014). Absolute and Relative Gene Expression in Listeria monocytogenes Using Real-Time PCR. In: Jordan, K., Fox, E., Wagner, M. (eds) Listeria monocytogenes. Methods in Molecular Biology, vol 1157. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0703-8_18
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DOI: https://doi.org/10.1007/978-1-4939-0703-8_18
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0702-1
Online ISBN: 978-1-4939-0703-8
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