Abstract
Receptor signaling can be evaluated in multiple ways, including analysis of phosphorylation of downstream molecules and analysis of proteins that are recruited to the receptor upon ligand binding. Majority of studies on the mechanism of DR3 signaling were performed using overexpression systems that can often lead to artifacts. In this chapter we describe how to analyze DR3 downstream events with most attention being paid to endogenous immunoprecipitation.
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References
Lavrik I, Golks A, Krammer PH (2005) Death receptor signaling. J Cell Sci 118:265–267
Bodmer JL, Burns K, Schneider P, Hofmann K, Steiner V, Thome M et al (1997) TRAMP, a novel apoptosis-mediating receptor with sequence homology to tumor necrosis factor receptor 1 and Fas(Apo-1/CD95). Immunity 6:79–88
Chinnaiyan AM, O'Rourke K, Yu GL, Lyons RH, Garg M, Duan DR et al (1996) Signal transduction by DR3, a death domain-containing receptor related to TNFR-1 and CD95. Science 274:990–992
Kitson J, Raven T, Jiang YP, Goeddel DV, Giles KM, Pun KT et al (1996) A death-domain-containing receptor that mediates apoptosis. Nature 384:372–375
Screaton GR, Xu XN, Olsen AL, Cowper AE, Tan R, McMichael AJ et al (1997) LARD: a new lymphoid-specific death domain containing receptor regulated by alternative pre-mRNA splicing. Proc Natl Acad Sci U S A 94:4615–4619
Tan KB, Harrop J, Reddy M, Young P, Terrett J, Emery J et al (1997) Characterization of a novel TNF-like ligand and recently described TNF ligand and TNF receptor superfamily genes and their constitutive and inducible expression in hematopoietic and non-hematopoietic cells. Gene 204:35–46
Migone TS, Zhang J, Luo X, Zhuang L, Chen C, Hu B et al (2002) TL1A is a TNF-like ligand for DR3 and TR6/DcR3 and functions as a T cell costimulator. Immunity 16:479–492
Bamias G, Martin C 3rd, Marini M, Hoang S, Mishina M, Ross WG et al (2003) Expression, localization, and functional activity of TL1A, a novel Th1-polarizing cytokine in inflammatory bowel disease. J Immunol 171:4868–4874
Bamias G, Mishina M, Nyce M, Ross WG, Kollias G, Rivera-Nieves J et al (2006) Role of TL1A and its receptor DR3 in two models of chronic murine ileitis. Proc Natl Acad Sci U S A 103:8441–8446
Fang L, Adkins B, Deyev V, Podack ER (2008) Essential role of TNF receptor superfamily 25 (TNFRSF25) in the development of allergic lung inflammation. J Exp Med 205:1037–1048
Meylan F, Davidson TS, Kahle E, Kinder M, Acharya K, Jankovic D et al (2008) The TNF-family receptor DR3 is essential for diverse T cell-mediated inflammatory diseases. Immunity 29:79–89
Pappu BP, Borodovsky A, Zheng TS, Yang X, Wu P, Dong X et al (2008) TL1A-DR3 interaction regulates Th17 cell function and Th17-mediated autoimmune disease. J Exp Med 205:1049–1062
Chen NJ, Chio II, Lin WJ, Duncan G, Chau H, Katz D et al (2008) Beyond tumor necrosis factor receptor: TRADD signaling in toll-like receptors. Proc Natl Acad Sci U S A 105:12429–12434
Pobezinskaya YL, Choksi S, Morgan MJ, Cao X, Liu ZG (2011) The adaptor protein TRADD is essential for TNF-like ligand 1A/death receptor 3 signaling. J Immunol 186:5212–5216
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Pobezinskaya, Y.L., Liu, Z., Choksi, S. (2014). Dissecting DR3 Signaling. In: Bayry, J. (eds) The TNF Superfamily. Methods in Molecular Biology, vol 1155. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0669-7_2
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DOI: https://doi.org/10.1007/978-1-4939-0669-7_2
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