Advertisement

Ovarian Tissue Cryopreservation and Transplantation: A Realistic, Effective Technology for Fertility Preservation

  • Dror Meirow
  • Hila Ra’anani
  • Hannah Biderman
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1154)

Abstract

It is clear that ovarian tissue cryopreservation can serve a very important role in providing fertility preservation. To date, more than 30 live human births have resulted from the transplantation of cryopreserved ovarian tissue, proving the successful implementation of this technique. The ideal conditions for ovarian tissue cryopreservation have yet to be determined, indicating the crucial need for more research in this field. Nonetheless, it is recommended that ovarian tissue cryopreservation be offered as an option to women before undergoing chemotherapy treatment in instances where there is no time to delay for hormonal stimulation and oocyte retrieval.

Key words

Ovarian tissue cryopreservation Fertility Transplantation 

References

  1. 1.
    Meirow D (2000) Reproduction post-chemotherapy in young cancer patients. Mol Cell Endocrinol 169(1–2):123–131PubMedCrossRefGoogle Scholar
  2. 2.
    ECotACSfRM (2005) Fertility preservation and reproduction in cancer patients. Fertil Steril 83:1622–1628CrossRefGoogle Scholar
  3. 3.
    Donnez J, Dolmans MM (2010) Cryopreservation and transplantation of ovarian tissue. Clin Obstet Gynecol 53(4):787–796Google Scholar
  4. 4.
    Meirow D, Schenker JG (1998) Cryopreservation and transplantation of ovarian tissue as a means of preserving female fertility. Harefuah 134(6):461–464PubMedGoogle Scholar
  5. 5.
    Meirow D (2010) Transplantation of cryopreserved ovarian tissues. Fertil Cryopreserv. In: Fertility Preservation, ed Ri Cheng Chian and Patrick Quinn. Published by Cambridge University Press. 218–232Google Scholar
  6. 6.
    Goldman MB (1974) Immunogenetic factors in the survival of ovarian transplants. Transplantation 17(5):518–523PubMedCrossRefGoogle Scholar
  7. 7.
    Nugent D, Meirow D, Brook PF, Aubard Y, Gosden RG (1997) Transplantation in reproductive medicine: previous experience, present knowledge and future prospects. Hum Reprod Update 3(3):267–280PubMedCrossRefGoogle Scholar
  8. 8.
    Gosden RG, Baird DT, Wade JC, Webb R (1994) Restoration of fertility to oophorectomized sheep by ovarian autografts stored at −196 degrees C. Hum Reprod 9(4):597–603PubMedGoogle Scholar
  9. 9.
    Baird DT, Webb R, Campbell BK, Harkness LM, Gosden RG (1999) Long-term ovarian function in sheep after ovariectomy and transplantation of autografts stored at −196 C. Endocrinology 140(1):462–471PubMedGoogle Scholar
  10. 10.
    Candy CJ, Wood MJ, Whittingham DG (1995) Follicular development in cryopreserved marmoset ovarian tissue after transplantation. Hum Reprod 10(9):2334–2338PubMedCrossRefGoogle Scholar
  11. 11.
    Salle B, Demirci B, Franck M, Rudigoz RC, Guerin JF, Lornage J (2002) Normal pregnancies and live births after autograft of frozen-thawed hemi-ovaries into ewes. Fertil Steril 77(2):403–408PubMedCrossRefGoogle Scholar
  12. 12.
    Almodin CG, Minguetti-Camara VC, Meister H et al (2004) Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy. Hum Reprod 19(6):1287–1293PubMedCrossRefGoogle Scholar
  13. 13.
    Dissen GA, Lara HE, Fahrenbach WH, Costa ME, Ojeda SR (1994) Immature rat ovaries become revascularized rapidly after autotransplantation and show a gonadotropin-dependent increase in angiogenic factor gene expression. Endocrinology 134(3):1146–1154PubMedGoogle Scholar
  14. 14.
    Van Eyck AS, Bouzin C, Feron O, Romeu L, Van Langendonckt A, Donnez J, Dolmans MM (2010) Both host and graft vessels contribute to revascularization of xenografted human ovarian tissue in a murine model. Fertil Steril 93(5):1676–1685PubMedCrossRefGoogle Scholar
  15. 15.
    Lee DM, Yeoman RR, Battaglia DE et al (2004) Live birth after ovarian tissue transplant. Nature 428(6979):137–138PubMedCrossRefGoogle Scholar
  16. 16.
    Oktay KNH, Mullan J, Gosden RG (1998) Development of human primordial follicles to antral stages in SCID/hpg mice stimulated with follicle stimulating hormone. Hum Reprod 13:1133–1138PubMedCrossRefGoogle Scholar
  17. 17.
    Gook DA, Edgar DH, Borg J, Archer J, Lutjen PJ, McBain JC (2003) Oocyte maturation, follicle rupture and luteinization in human cryopreserved ovarian tissue following xenografting. Hum Reprod 18(9):1772–1781PubMedCrossRefGoogle Scholar
  18. 18.
    Gook DA, Edgar DH, Borg J, Archer J, McBain JC (2005) Diagnostic assessment of the developmental potential of human cryopreserved ovarian tissue from multiple patients using xenografting. Hum Reprod 20(1):72–78PubMedGoogle Scholar
  19. 19.
    Kim SS, Soules MR, Battaglia DE (2002) Follicular development, ovulation, and corpus luteum formation in cryopreserved human ovarian tissue after xenotransplantation. Fertil Steril 78(1):77–82PubMedCrossRefGoogle Scholar
  20. 20.
    Meirow D, Fasouliotis SJ, Nugent D, Schenker JG, Gosden RG, Rutherford AJ (1999) A laparoscopic technique for obtaining ovarian cortical biopsy specimens for fertility conservation in patients with cancer. Fertil Steril 71(5):948–951PubMedCrossRefGoogle Scholar
  21. 21.
    Meirow D, Baum M, Yaron R et al (2007) Ovarian tissue cryopreservation in hematologic malignancy: ten years’ experience. Leuk Lymphoma 48(8):1569–1576PubMedCrossRefGoogle Scholar
  22. 22.
    Cobo A, Meseguer M, Remohi J, Pellicer A (2010) Use of cryo-banked oocytes in an ovum donation programme: a prospective, randomized, controlled, clinical trial. Hum Reprod 25(9):2239–2246PubMedCrossRefGoogle Scholar
  23. 23.
    Meirow D, Schiff E (2005) Impact of maternal exposure to chemotherapy on embryos and offspring J National Cancer Institute 34:21–25Google Scholar
  24. 24.
    Kujjo LL, Chang EA, Pereira RJ et al (2011) Chemotherapy-induced late transgenerational effects in mice. PLoS One 6(3):e17877PubMedCentralPubMedCrossRefGoogle Scholar
  25. 25.
    Bar-Joseph H, Ben-Aharon I, Rizel S, Stemmer SM, Tzabari M, Shalgi R (2010) Doxorubicin-induced apoptosis in germinal vesicle (GV) oocytes. Reprod Toxicol 30(4):566–572PubMedCrossRefGoogle Scholar
  26. 26.
    Winther JF, Boice JD Jr, Mulvihill JJ et al (2004) Chromosomal abnormalities among offspring of childhood-cancer survivors in Denmark: a population-based study. Am J Hum Genet 74(6):1282–1285PubMedCentralPubMedCrossRefGoogle Scholar
  27. 27.
    Donnez J, Dolmans MM, Demylle D et al (2004) Livebirth after orthotopic transplantation of cryopreserved ovarian tissue. Lancet 364(9443):1405–1410PubMedCrossRefGoogle Scholar
  28. 28.
    Donnez J, Squifflet J, Dolmans M (2011) Ovarian tissue transplantation. In: Donnez J, Samuel Kim S (eds) Principles and practice of fertility preservation. Cambridge University Press, New York, pp 357–366CrossRefGoogle Scholar
  29. 29.
    Borgstrom B, Hreinsson J, Rasmussen C et al (2009) Fertility preservation in girls with turner syndrome: prognostic signs of the presence of ovarian follicles. J Clin Endocrinol Metab 94(1):74–80PubMedCrossRefGoogle Scholar
  30. 30.
    Andersen CY, Rosendahl M, Byskov AG et al (2008) Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue. Hum Reprod 23(10):2266–2272PubMedCrossRefGoogle Scholar
  31. 31.
    Meirow D, Levron J, Eldar-Geva T et al (2005) Pregnancy after transplantation of cryopreserved ovarian tissue in a patient with ovarian failure after chemotherapy. N Engl J Med 353(3):318–321PubMedCrossRefGoogle Scholar
  32. 32.
    Donnez J, Silber S, Andersen CY et al (2010) Children born after autotransplantation of cryopreserved ovarian tissue. a review of 13 live births. Ann Med 43(6):437–450CrossRefGoogle Scholar
  33. 33.
    Meirow D, Fasouliotis SJ, Nugent D, Schenker JG, Gosden RG, Rutherford AJ (1999) A laparoscopic technique for obtaining ovarian cortical biopsies for fertility conservation in cancer patients. Fertil Steril 71:948–951PubMedCrossRefGoogle Scholar
  34. 34.
    Revel A, Koler M, Simon A, Lewin A, Laufer N, Safran A (2003) Oocyte collection during cryopreservation of the ovarian cortex. Fertil Steril 79(5):1237–1239PubMedCrossRefGoogle Scholar
  35. 35.
    Seshadri T, Gook D, Lade S et al (2006) Lack of evidence of disease contamination in ovarian tissue harvested for cryopreservation from patients with Hodgkin lymphoma and analysis of factors predictive of oocyte yield. Br J Cancer 94(7):1007–1010PubMedCentralPubMedCrossRefGoogle Scholar
  36. 36.
    Gook D, Edgar DH (2011) Ovarian tissue cryopreservation. In: Donnez J, Samuel Kim S (eds) Principles and practice of fertility preservation. Cambridge University Press, New York, pp 342–356CrossRefGoogle Scholar
  37. 37.
    Schmidt KL, Ernst E, Byskov AG, Nyboe Andersen A, Yding Andersen C (2003) Survival of primordial follicles following prolonged transportation of ovarian tissue prior to cryopreservation. Hum Reprod 18(12):2654–2659PubMedCrossRefGoogle Scholar
  38. 38.
    Mazur P (1970) Cryobiology: the freezing of biological systems. Science 168(3934):939–949PubMedCrossRefGoogle Scholar
  39. 39.
    Hunter J, Bernard A, Fuller B, McGrath J, Shaw RW (1992) Plasma membrane water permeabilities of human oocytes: the temperature dependence of water movement in individual cells. J Cell Physiol 150(1):175–179PubMedCrossRefGoogle Scholar
  40. 40.
    Newton H, Fisher J, Arnold JR, Pegg DE, Faddy MJ, Gosden RG (1998) Permeation of human ovarian tissue with cryoprotective agents in preparation for cryopreservation. Hum Reprod 13(2):376–380PubMedCrossRefGoogle Scholar
  41. 41.
    Oktay K, Nugent D, Newton H, Salha O, Chatterjee P, Gosden RG (1997) Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue. Fertil Steril 67(3):481–486PubMedCrossRefGoogle Scholar
  42. 42.
    Picton HM, Kim SS, Gosden RG (2000) Cryopreservation of gonadal tissue and cells. Br Med Bull 56(3):603–615PubMedCrossRefGoogle Scholar
  43. 43.
    Gook DA, Edgar DH, Stern C (1999) Effect of cooling rate and dehydration regimen on the histological appearance of human ovarian cortex following cryopreservation in 1, 2-propanediol. Hum Reprod 14(8):2061–2068PubMedCrossRefGoogle Scholar
  44. 44.
    Meirow D, Levron J, Eldar-Geva T et al (2007) Monitoring the ovaries after autotransplantation of cryopreserved ovarian tissue: endocrine studies, in vitro fertilization cycles, and live birth. Fertil Steril 87(2):418 e7–418 e15CrossRefGoogle Scholar
  45. 45.
    Kagawa N, Silber S, Kuwayama M (2009) Successful vitrification of bovine and human ovarian tissue. Reprod Biomed Online 18(4):568–577PubMedCrossRefGoogle Scholar
  46. 46.
    Fahy GM, Wowk B, Wu J, Paynter S (2004) Improved vitrification solutions based on the predictability of vitrification solution toxicity. Cryobiology 48(1):22–35PubMedCrossRefGoogle Scholar
  47. 47.
    Keros V, Xella S, Hultenby K et al (2009) Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue. Hum Reprod 24(7):1670–1683PubMedCrossRefGoogle Scholar
  48. 48.
    Meirow D, Hardan I, Dor J et al (2008) Searching for evidence of disease and malignant cell contamination in ovarian tissue stored from hematologic cancer patients. Hum Reprod 23(5):1007–1013PubMedCrossRefGoogle Scholar
  49. 49.
    Dolmans MM, Marinescu C, Saussoy P et al (2010) Reimplantation of cryopreserved ovarian tissue from patients with acute lymphoblastic leukemia is potentially unsafe. Blood 116(16):2908–2914Google Scholar
  50. 50.
    Rosendahl MAM, Ralfkiaer E et al (2010) Evidence of residual disease in cryopreserved ovarian cortex from female patients with leukemia. Fertil Steril 94:2186–2190PubMedCrossRefGoogle Scholar
  51. 51.
    Demeestere I, Simon P, Emiliani S, Delbaere A, Englert Y (2006) Options to preserve fertility before oncological treatment: cryopreservation of ovarian tissue and its clinical application. Acta Clin Belg 61(5):259–263PubMedCrossRefGoogle Scholar
  52. 52.
    Demeestere I, Simon P, Emiliani S, Delbaere A, Englert Y (2009) Orthotopic and heterotopic ovarian tissue transplantation. Hum Reprod Update 15(6):649–665PubMedCentralPubMedCrossRefGoogle Scholar
  53. 53.
    Silber SJ, Lenahan KM, Levine DJ et al (2005) Ovarian transplantation between monozygotic twins discordant for premature ovarian failure. N Engl J Med 353(1):58–63PubMedCrossRefGoogle Scholar
  54. 54.
    Demeestere I, Simon P, Buxant F et al (2006) Ovarian function and spontaneous pregnancy after combined heterotopic and orthotopic cryopreserved ovarian tissue transplantation in a patient previously treated with bone marrow transplantation: case report. Hum Reprod 21(8):2010–2014PubMedCrossRefGoogle Scholar
  55. 55.
    Piver P, Amiot C, Agnani G et al (2009) Two pregnancies obtained after a new technique of autotranplantation of cryopreserved ovarian tissue. Hum Reprod 24:i150-035Google Scholar
  56. 56.
    Revel A, Laufer N, Ben Meir A, Lebovich M, Mitrani E (2011) Micro-organ ovarian transplantation enables pregnancy: a case report. Hum Reprod 26(5):1097–1103PubMedCrossRefGoogle Scholar
  57. 57.
    Nugent D, Newton H, Gallivan L, Gosden RG (1998) Protective effect of vitamin E on ischaemia-reperfusion injury in ovarian grafts. J Reprod Fertil 114(2):341–346PubMedCrossRefGoogle Scholar
  58. 58.
    Maltaris T, Beckmann MW, Binder H et al (2007) The effect of a GnRH agonist on cryopreserved human ovarian grafts in severe combined immunodeficient mice. Reproduction 133(2):503–509PubMedCrossRefGoogle Scholar
  59. 59.
    Sanchez-Serrano M, Crespo J, Mirabet V et al (2010) Twins born after transplantation of ovarian cortical tissue and oocyte vitrification. Fertil Steril 93(1):268 e11–268 e13CrossRefGoogle Scholar
  60. 60.
    Ernst E, Bergholdt S, Jorgensen JS, Andersen CY (2010) The first woman to give birth to two children following transplantation of frozen/thawed ovarian tissue. Hum Reprod 25(5):1280–1281PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Fertility Preservation center and IVF Unit, Sheba Medical Center Tel-HashomerSackler School of Medicine, Tel-Aviv UniversityTel AvivIsrael

Personalised recommendations