Abstract
It is clear that ovarian tissue cryopreservation can serve a very important role in providing fertility preservation. To date, more than 30 live human births have resulted from the transplantation of cryopreserved ovarian tissue, proving the successful implementation of this technique. The ideal conditions for ovarian tissue cryopreservation have yet to be determined, indicating the crucial need for more research in this field. Nonetheless, it is recommended that ovarian tissue cryopreservation be offered as an option to women before undergoing chemotherapy treatment in instances where there is no time to delay for hormonal stimulation and oocyte retrieval.
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Meirow D (2000) Reproduction post-chemotherapy in young cancer patients. Mol Cell Endocrinol 169(1–2):123–131
ECotACSfRM (2005) Fertility preservation and reproduction in cancer patients. Fertil Steril 83:1622–1628
Donnez J, Dolmans MM (2010) Cryopreservation and transplantation of ovarian tissue. Clin Obstet Gynecol 53(4):787–796
Meirow D, Schenker JG (1998) Cryopreservation and transplantation of ovarian tissue as a means of preserving female fertility. Harefuah 134(6):461–464
Meirow D (2010) Transplantation of cryopreserved ovarian tissues. Fertil Cryopreserv. In: Fertility Preservation, ed Ri Cheng Chian and Patrick Quinn. Published by Cambridge University Press. 218–232
Goldman MB (1974) Immunogenetic factors in the survival of ovarian transplants. Transplantation 17(5):518–523
Nugent D, Meirow D, Brook PF, Aubard Y, Gosden RG (1997) Transplantation in reproductive medicine: previous experience, present knowledge and future prospects. Hum Reprod Update 3(3):267–280
Gosden RG, Baird DT, Wade JC, Webb R (1994) Restoration of fertility to oophorectomized sheep by ovarian autografts stored at −196 degrees C. Hum Reprod 9(4):597–603
Baird DT, Webb R, Campbell BK, Harkness LM, Gosden RG (1999) Long-term ovarian function in sheep after ovariectomy and transplantation of autografts stored at −196 C. Endocrinology 140(1):462–471
Candy CJ, Wood MJ, Whittingham DG (1995) Follicular development in cryopreserved marmoset ovarian tissue after transplantation. Hum Reprod 10(9):2334–2338
Salle B, Demirci B, Franck M, Rudigoz RC, Guerin JF, Lornage J (2002) Normal pregnancies and live births after autograft of frozen-thawed hemi-ovaries into ewes. Fertil Steril 77(2):403–408
Almodin CG, Minguetti-Camara VC, Meister H et al (2004) Recovery of fertility after grafting of cryopreserved germinative tissue in female rabbits following radiotherapy. Hum Reprod 19(6):1287–1293
Dissen GA, Lara HE, Fahrenbach WH, Costa ME, Ojeda SR (1994) Immature rat ovaries become revascularized rapidly after autotransplantation and show a gonadotropin-dependent increase in angiogenic factor gene expression. Endocrinology 134(3):1146–1154
Van Eyck AS, Bouzin C, Feron O, Romeu L, Van Langendonckt A, Donnez J, Dolmans MM (2010) Both host and graft vessels contribute to revascularization of xenografted human ovarian tissue in a murine model. Fertil Steril 93(5):1676–1685
Lee DM, Yeoman RR, Battaglia DE et al (2004) Live birth after ovarian tissue transplant. Nature 428(6979):137–138
Oktay KNH, Mullan J, Gosden RG (1998) Development of human primordial follicles to antral stages in SCID/hpg mice stimulated with follicle stimulating hormone. Hum Reprod 13:1133–1138
Gook DA, Edgar DH, Borg J, Archer J, Lutjen PJ, McBain JC (2003) Oocyte maturation, follicle rupture and luteinization in human cryopreserved ovarian tissue following xenografting. Hum Reprod 18(9):1772–1781
Gook DA, Edgar DH, Borg J, Archer J, McBain JC (2005) Diagnostic assessment of the developmental potential of human cryopreserved ovarian tissue from multiple patients using xenografting. Hum Reprod 20(1):72–78
Kim SS, Soules MR, Battaglia DE (2002) Follicular development, ovulation, and corpus luteum formation in cryopreserved human ovarian tissue after xenotransplantation. Fertil Steril 78(1):77–82
Meirow D, Fasouliotis SJ, Nugent D, Schenker JG, Gosden RG, Rutherford AJ (1999) A laparoscopic technique for obtaining ovarian cortical biopsy specimens for fertility conservation in patients with cancer. Fertil Steril 71(5):948–951
Meirow D, Baum M, Yaron R et al (2007) Ovarian tissue cryopreservation in hematologic malignancy: ten years’ experience. Leuk Lymphoma 48(8):1569–1576
Cobo A, Meseguer M, Remohi J, Pellicer A (2010) Use of cryo-banked oocytes in an ovum donation programme: a prospective, randomized, controlled, clinical trial. Hum Reprod 25(9):2239–2246
Meirow D, Schiff E (2005) Impact of maternal exposure to chemotherapy on embryos and offspring J National Cancer Institute 34:21–25
Kujjo LL, Chang EA, Pereira RJ et al (2011) Chemotherapy-induced late transgenerational effects in mice. PLoS One 6(3):e17877
Bar-Joseph H, Ben-Aharon I, Rizel S, Stemmer SM, Tzabari M, Shalgi R (2010) Doxorubicin-induced apoptosis in germinal vesicle (GV) oocytes. Reprod Toxicol 30(4):566–572
Winther JF, Boice JD Jr, Mulvihill JJ et al (2004) Chromosomal abnormalities among offspring of childhood-cancer survivors in Denmark: a population-based study. Am J Hum Genet 74(6):1282–1285
Donnez J, Dolmans MM, Demylle D et al (2004) Livebirth after orthotopic transplantation of cryopreserved ovarian tissue. Lancet 364(9443):1405–1410
Donnez J, Squifflet J, Dolmans M (2011) Ovarian tissue transplantation. In: Donnez J, Samuel Kim S (eds) Principles and practice of fertility preservation. Cambridge University Press, New York, pp 357–366
Borgstrom B, Hreinsson J, Rasmussen C et al (2009) Fertility preservation in girls with turner syndrome: prognostic signs of the presence of ovarian follicles. J Clin Endocrinol Metab 94(1):74–80
Andersen CY, Rosendahl M, Byskov AG et al (2008) Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue. Hum Reprod 23(10):2266–2272
Meirow D, Levron J, Eldar-Geva T et al (2005) Pregnancy after transplantation of cryopreserved ovarian tissue in a patient with ovarian failure after chemotherapy. N Engl J Med 353(3):318–321
Donnez J, Silber S, Andersen CY et al (2010) Children born after autotransplantation of cryopreserved ovarian tissue. a review of 13 live births. Ann Med 43(6):437–450
Meirow D, Fasouliotis SJ, Nugent D, Schenker JG, Gosden RG, Rutherford AJ (1999) A laparoscopic technique for obtaining ovarian cortical biopsies for fertility conservation in cancer patients. Fertil Steril 71:948–951
Revel A, Koler M, Simon A, Lewin A, Laufer N, Safran A (2003) Oocyte collection during cryopreservation of the ovarian cortex. Fertil Steril 79(5):1237–1239
Seshadri T, Gook D, Lade S et al (2006) Lack of evidence of disease contamination in ovarian tissue harvested for cryopreservation from patients with Hodgkin lymphoma and analysis of factors predictive of oocyte yield. Br J Cancer 94(7):1007–1010
Gook D, Edgar DH (2011) Ovarian tissue cryopreservation. In: Donnez J, Samuel Kim S (eds) Principles and practice of fertility preservation. Cambridge University Press, New York, pp 342–356
Schmidt KL, Ernst E, Byskov AG, Nyboe Andersen A, Yding Andersen C (2003) Survival of primordial follicles following prolonged transportation of ovarian tissue prior to cryopreservation. Hum Reprod 18(12):2654–2659
Mazur P (1970) Cryobiology: the freezing of biological systems. Science 168(3934):939–949
Hunter J, Bernard A, Fuller B, McGrath J, Shaw RW (1992) Plasma membrane water permeabilities of human oocytes: the temperature dependence of water movement in individual cells. J Cell Physiol 150(1):175–179
Newton H, Fisher J, Arnold JR, Pegg DE, Faddy MJ, Gosden RG (1998) Permeation of human ovarian tissue with cryoprotective agents in preparation for cryopreservation. Hum Reprod 13(2):376–380
Oktay K, Nugent D, Newton H, Salha O, Chatterjee P, Gosden RG (1997) Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue. Fertil Steril 67(3):481–486
Picton HM, Kim SS, Gosden RG (2000) Cryopreservation of gonadal tissue and cells. Br Med Bull 56(3):603–615
Gook DA, Edgar DH, Stern C (1999) Effect of cooling rate and dehydration regimen on the histological appearance of human ovarian cortex following cryopreservation in 1, 2-propanediol. Hum Reprod 14(8):2061–2068
Meirow D, Levron J, Eldar-Geva T et al (2007) Monitoring the ovaries after autotransplantation of cryopreserved ovarian tissue: endocrine studies, in vitro fertilization cycles, and live birth. Fertil Steril 87(2):418 e7–418 e15
Kagawa N, Silber S, Kuwayama M (2009) Successful vitrification of bovine and human ovarian tissue. Reprod Biomed Online 18(4):568–577
Fahy GM, Wowk B, Wu J, Paynter S (2004) Improved vitrification solutions based on the predictability of vitrification solution toxicity. Cryobiology 48(1):22–35
Keros V, Xella S, Hultenby K et al (2009) Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue. Hum Reprod 24(7):1670–1683
Meirow D, Hardan I, Dor J et al (2008) Searching for evidence of disease and malignant cell contamination in ovarian tissue stored from hematologic cancer patients. Hum Reprod 23(5):1007–1013
Dolmans MM, Marinescu C, Saussoy P et al (2010) Reimplantation of cryopreserved ovarian tissue from patients with acute lymphoblastic leukemia is potentially unsafe. Blood 116(16):2908–2914
Rosendahl MAM, Ralfkiaer E et al (2010) Evidence of residual disease in cryopreserved ovarian cortex from female patients with leukemia. Fertil Steril 94:2186–2190
Demeestere I, Simon P, Emiliani S, Delbaere A, Englert Y (2006) Options to preserve fertility before oncological treatment: cryopreservation of ovarian tissue and its clinical application. Acta Clin Belg 61(5):259–263
Demeestere I, Simon P, Emiliani S, Delbaere A, Englert Y (2009) Orthotopic and heterotopic ovarian tissue transplantation. Hum Reprod Update 15(6):649–665
Silber SJ, Lenahan KM, Levine DJ et al (2005) Ovarian transplantation between monozygotic twins discordant for premature ovarian failure. N Engl J Med 353(1):58–63
Demeestere I, Simon P, Buxant F et al (2006) Ovarian function and spontaneous pregnancy after combined heterotopic and orthotopic cryopreserved ovarian tissue transplantation in a patient previously treated with bone marrow transplantation: case report. Hum Reprod 21(8):2010–2014
Piver P, Amiot C, Agnani G et al (2009) Two pregnancies obtained after a new technique of autotranplantation of cryopreserved ovarian tissue. Hum Reprod 24:i150-035
Revel A, Laufer N, Ben Meir A, Lebovich M, Mitrani E (2011) Micro-organ ovarian transplantation enables pregnancy: a case report. Hum Reprod 26(5):1097–1103
Nugent D, Newton H, Gallivan L, Gosden RG (1998) Protective effect of vitamin E on ischaemia-reperfusion injury in ovarian grafts. J Reprod Fertil 114(2):341–346
Maltaris T, Beckmann MW, Binder H et al (2007) The effect of a GnRH agonist on cryopreserved human ovarian grafts in severe combined immunodeficient mice. Reproduction 133(2):503–509
Sanchez-Serrano M, Crespo J, Mirabet V et al (2010) Twins born after transplantation of ovarian cortical tissue and oocyte vitrification. Fertil Steril 93(1):268 e11–268 e13
Ernst E, Bergholdt S, Jorgensen JS, Andersen CY (2010) The first woman to give birth to two children following transplantation of frozen/thawed ovarian tissue. Hum Reprod 25(5):1280–1281
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Meirow, D., Ra’anani, H., Biderman, H. (2014). Ovarian Tissue Cryopreservation and Transplantation: A Realistic, Effective Technology for Fertility Preservation. In: Rosenwaks, Z., Wassarman, P. (eds) Human Fertility. Methods in Molecular Biology, vol 1154. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0659-8_21
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DOI: https://doi.org/10.1007/978-1-4939-0659-8_21
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