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Techniques for Slow Cryopreservation of Embryos

  • Lucinda Veeck Gosden
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1154)

Abstract

The slow cryopreservation of embryos has been used for nearly three decades as a means of storing surplus conceptuses from single IVF (in vitro fertilization) cycles. Doing so has allowed caregivers to maximize pregnancy rates without wastage of precious biological materials. Very detailed methods are described here using a popular biological freezing unit manufactured by Planer PLC (Middlesex, UK). Culture media preparation and tranfer protocols, including replacement in both natural and stimulated cycles, are included.

Key words

Cryopreservation Thawing Prouclear stage oocyte Embryo Blastocyst Slow freezing 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Weill Cornell Medical CollegeNew YorkUSA

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