Oocyte Retrieval and Quality Evaluation

  • Lucinda Veeck GosdenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1154)


After more than 30 years of harvesting oocytes for in vitro fertilization, collection techniques are well established. Presented here are dependable methods for maintaining environmental stability during such procedures and general descriptions of the materials collected.

Key words

Oocyte Metaphase I Metaphase II Prophase I Granulosa Ooplasm Oolemma Zona pellucida 


  1. 1.
    Sathananthan AH, Trounson AO, Wood C (1986) Atlas of fine structure of human sperm penetration, eggs, and embryos cultured in vitro. Praeger, New York, p 2 (penetration of immature oocytes); p 4 (chromosomes and spindle microtubules); p 10 (cortical granules); p 42, p 126 (oocyte ageing); p 90 (polar body nucleus)Google Scholar
  2. 2.
    Van Blerkom J, Davis PW, Merriam J (1994) A retrospective analysis of unfertilized and presumed parthenogenetically activated human oocytes demonstrates a high frequency of sperm penetration. Hum Reprod 9:2381PubMedGoogle Scholar
  3. 3.
    Edwards RG (1980) Conception in the human female. Academic, New York, p 634Google Scholar
  4. 4.
    Dekel N, Beers WH (1980) Development of the rat oocyte in vitro: inhibition and induction of maturation in the presence or absence of the cumulus oophorus. Dev Biol 75:247PubMedCrossRefGoogle Scholar
  5. 5.
    Gregory L, Booth AD, Wells C et al (1994) A study of the cumulus-corona cell complex in in-vitro fertilization and embryo transfer; a prognostic indicator of the failure of implantation. Hum Reprod 9:1308PubMedGoogle Scholar
  6. 6.
    Veeck LL (1988) Oocyte assessment and biological performance. Ann N Y Acad Sci 541:259CrossRefGoogle Scholar
  7. 7.
    Veeck LL (1985) Extracorporeal maturation, Norfolk, Virginia. Ann N Y Acad Sci 442:357PubMedCrossRefGoogle Scholar
  8. 8.
    Veeck LL (1986) The morphologic estimation of mature oocytes and their preparation for insemination. In: Jones HW Jr, Jones GS, Hodgen GD, Rosenwaks Z (eds) In vitro fertilization—Norfolk. Williams & Wilkins, Baltimore, p 81Google Scholar
  9. 9.
    Hammitt DG, Syrop CH, Van Voorhis BJ et al (1993) Maturational asynchrony between oocyte cumulus-coronal morphology and nuclear maturity in gonadotropin-releasing hormone agonist stimulations. Fertil Steril 59:375PubMedGoogle Scholar
  10. 10.
    Laufer N, Tarlatzis BC, DeCherney AH et al (1984) Asynchrony between human cumulus-corona cell complex and oocyte maturation after human menopausal gonadotropin treatment for in vitro fertilization. Fertil Steril 42:366PubMedGoogle Scholar
  11. 11.
    Veeck LL (1986) Atlas of the human oocyte and early conceptus, vol 1. Williams & Wilkins, Baltimore, p 7, p 127 (mature granulosa); p 57, p 68 (oocyte classification); p 74 (disparity between cumulus and nucleus)Google Scholar
  12. 12.
    Veeck LL (1991) Atlas of the human oocyte and early conceptus, vol 2. Williams & Wilkins, Baltimore, p 3, p 13, p 27 (membrana granulosa); p 13 (oocyte classification); p 27 (disparity between cumulus and nucleus)Google Scholar
  13. 13.
    Veeck LL (1990) Pregnancy rate and pregnancy outcome associated with laboratory evaluation of spermatozoa, oocytes, and preembryos. In: Mashiach S, Ben-Rafael Z, Laufer N, Schenker JG (eds) Advances in assisted reproductive technologies. Plenum Press, New York, p 745CrossRefGoogle Scholar
  14. 14.
    Veeck LL (1990) The morphological assessment of human oocytes and early concepti. In: Keel BA, Webster BW (eds) Handbook of the laboratory diagnosis and treatment of infertility. CRC Press, Boca Raton, p 353Google Scholar
  15. 15.
    Coetzee K, Windt ML (1996) Fertilization and pregnancy using metaphase I oocytes in an intracytoplasmic sperm injection program. J Assist Reprod Genet 13:768PubMedCrossRefGoogle Scholar
  16. 16.
    Barnes FL, Crombie A, Gardner DK et al (1995) Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection, and assisted hatching. Hum Reprod 10:3243Google Scholar
  17. 17.
    Cha KY, Koo JJ, Choi DH et al (1991) Pregnancy after in vitro fertilization of human follicular oocytes collected from non-stimulated cycles, their culture in vitro, and their transfer in a donor oocyte program. Fertil Steril 55:109PubMedGoogle Scholar
  18. 18.
    Liu J, Katz E, Garcia JE et al (1997) Successful in vitro maturation of human oocytes not exposed to human chorionic gonadotropin during ovulation induction, resulting in pregnancy. Fertil Steril 67:566CrossRefGoogle Scholar
  19. 19.
    Veeck LL, Wortham JWE, Witmyer J et al (1983) Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization. Fertil Steril 39:594PubMedGoogle Scholar
  20. 20.
    Austin CR (1961) The mammalian egg. Blackwell Press, Oxford, 75Google Scholar
  21. 21.
    Planchot M, Crozet N (1992) Fertilization abnormalities in human in vitro fertilization. Hum Reprod 7 suppl 1:89Google Scholar
  22. 22.
    Van Blerkom J, Henry G (1992) Oocyte dysmorphism and aneuploidy in meiotically mature human oocytes after ovarian stimulation. Hum Reprod 7:379–390PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Weill Cornell Medical CollegeNew YorkUSA

Personalised recommendations