Skip to main content

Oocyte Retrieval and Quality Evaluation

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1154))

Abstract

After more than 30 years of harvesting oocytes for in vitro fertilization, collection techniques are well established. Presented here are dependable methods for maintaining environmental stability during such procedures and general descriptions of the materials collected.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Sathananthan AH, Trounson AO, Wood C (1986) Atlas of fine structure of human sperm penetration, eggs, and embryos cultured in vitro. Praeger, New York, p 2 (penetration of immature oocytes); p 4 (chromosomes and spindle microtubules); p 10 (cortical granules); p 42, p 126 (oocyte ageing); p 90 (polar body nucleus)

    Google Scholar 

  2. Van Blerkom J, Davis PW, Merriam J (1994) A retrospective analysis of unfertilized and presumed parthenogenetically activated human oocytes demonstrates a high frequency of sperm penetration. Hum Reprod 9:2381

    PubMed  Google Scholar 

  3. Edwards RG (1980) Conception in the human female. Academic, New York, p 634

    Google Scholar 

  4. Dekel N, Beers WH (1980) Development of the rat oocyte in vitro: inhibition and induction of maturation in the presence or absence of the cumulus oophorus. Dev Biol 75:247

    Article  CAS  PubMed  Google Scholar 

  5. Gregory L, Booth AD, Wells C et al (1994) A study of the cumulus-corona cell complex in in-vitro fertilization and embryo transfer; a prognostic indicator of the failure of implantation. Hum Reprod 9:1308

    CAS  PubMed  Google Scholar 

  6. Veeck LL (1988) Oocyte assessment and biological performance. Ann N Y Acad Sci 541:259

    Article  CAS  Google Scholar 

  7. Veeck LL (1985) Extracorporeal maturation, Norfolk, Virginia. Ann N Y Acad Sci 442:357

    Article  CAS  PubMed  Google Scholar 

  8. Veeck LL (1986) The morphologic estimation of mature oocytes and their preparation for insemination. In: Jones HW Jr, Jones GS, Hodgen GD, Rosenwaks Z (eds) In vitro fertilization—Norfolk. Williams & Wilkins, Baltimore, p 81

    Google Scholar 

  9. Hammitt DG, Syrop CH, Van Voorhis BJ et al (1993) Maturational asynchrony between oocyte cumulus-coronal morphology and nuclear maturity in gonadotropin-releasing hormone agonist stimulations. Fertil Steril 59:375

    CAS  PubMed  Google Scholar 

  10. Laufer N, Tarlatzis BC, DeCherney AH et al (1984) Asynchrony between human cumulus-corona cell complex and oocyte maturation after human menopausal gonadotropin treatment for in vitro fertilization. Fertil Steril 42:366

    CAS  PubMed  Google Scholar 

  11. Veeck LL (1986) Atlas of the human oocyte and early conceptus, vol 1. Williams & Wilkins, Baltimore, p 7, p 127 (mature granulosa); p 57, p 68 (oocyte classification); p 74 (disparity between cumulus and nucleus)

    Google Scholar 

  12. Veeck LL (1991) Atlas of the human oocyte and early conceptus, vol 2. Williams & Wilkins, Baltimore, p 3, p 13, p 27 (membrana granulosa); p 13 (oocyte classification); p 27 (disparity between cumulus and nucleus)

    Google Scholar 

  13. Veeck LL (1990) Pregnancy rate and pregnancy outcome associated with laboratory evaluation of spermatozoa, oocytes, and preembryos. In: Mashiach S, Ben-Rafael Z, Laufer N, Schenker JG (eds) Advances in assisted reproductive technologies. Plenum Press, New York, p 745

    Chapter  Google Scholar 

  14. Veeck LL (1990) The morphological assessment of human oocytes and early concepti. In: Keel BA, Webster BW (eds) Handbook of the laboratory diagnosis and treatment of infertility. CRC Press, Boca Raton, p 353

    Google Scholar 

  15. Coetzee K, Windt ML (1996) Fertilization and pregnancy using metaphase I oocytes in an intracytoplasmic sperm injection program. J Assist Reprod Genet 13:768

    Article  CAS  PubMed  Google Scholar 

  16. Barnes FL, Crombie A, Gardner DK et al (1995) Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection, and assisted hatching. Hum Reprod 10:3243

    CAS  Google Scholar 

  17. Cha KY, Koo JJ, Choi DH et al (1991) Pregnancy after in vitro fertilization of human follicular oocytes collected from non-stimulated cycles, their culture in vitro, and their transfer in a donor oocyte program. Fertil Steril 55:109

    CAS  PubMed  Google Scholar 

  18. Liu J, Katz E, Garcia JE et al (1997) Successful in vitro maturation of human oocytes not exposed to human chorionic gonadotropin during ovulation induction, resulting in pregnancy. Fertil Steril 67:566

    Article  CAS  Google Scholar 

  19. Veeck LL, Wortham JWE, Witmyer J et al (1983) Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization. Fertil Steril 39:594

    CAS  PubMed  Google Scholar 

  20. Austin CR (1961) The mammalian egg. Blackwell Press, Oxford, 75

    Google Scholar 

  21. Planchot M, Crozet N (1992) Fertilization abnormalities in human in vitro fertilization. Hum Reprod 7 suppl 1:89

    Google Scholar 

  22. Van Blerkom J, Henry G (1992) Oocyte dysmorphism and aneuploidy in meiotically mature human oocytes after ovarian stimulation. Hum Reprod 7:379–390

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Lucinda Veeck Gosden D.Sc. (Hon) .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media New York

About this protocol

Cite this protocol

Gosden, L.V. (2014). Oocyte Retrieval and Quality Evaluation. In: Rosenwaks, Z., Wassarman, P. (eds) Human Fertility. Methods in Molecular Biology, vol 1154. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0659-8_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-0659-8_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0658-1

  • Online ISBN: 978-1-4939-0659-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics