Skip to main content
SpringerLink
Log in

Analysis of Plant Polyisoprenoids

  • Protocol
  • First Online:
Book cover Plant Isoprenoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1153))

Abstract

Polyisoprenoid alcohols are representatives of high-molecular terpenoids. Their hydrocarbon chains are built of 5 to more than 100 isoprene units giving rise to polymer molecules that differ in chain-length and/or geometrical configuration. Plants have been shown to accumulate diverse polyisoprenoid mixtures with tissue-specific composition. In this chapter, methods of analysis of polyisoprenoid alcohols in plant material are described, including isolation and purification of polyisoprenoids from plant tissue, fast semiquantitative analysis of the polyisoprenoid profile by thin-layer chromatography (straight phase adsorption and reversed phase partition techniques), and quantification of polyisoprenoids with the aid of high performance liquid chromatography. This approach results in full characterization of complex polyisoprenoid mixtures accumulated in various plant tissues and other matrixes.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Swiezewska E, Danikiewicz W (2005) Polyisoprenoids: structure, biosynthesis and function. Prog Lipid Res 44:235–258

    Article  PubMed  CAS  Google Scholar 

  2. Jozwiak A, Ples M, Skorupinska-Tudek K, Kania M, Dydak M, Danikiewicz W, Swiezewska E (2013) Sugar availability modulates polyisoprenoid and phytosterol profiles in Arabidopsis thaliana hairy root culture. Biochim Biophys Acta 1831:438–447

    Article  PubMed  CAS  Google Scholar 

  3. Ciepichal E, Wojcik J, Bienkowski T, Kania M, Swist M, Danikiewicz W, Marczewski A, Hertel J, Matysiak Z, Swiezewska E, Chojnacki T (2007) Alloprenols: novel α-trans-polyprenols of Allophylus caudatus. Chem Phys Lipids 147:103–112

    Article  PubMed  CAS  Google Scholar 

  4. Surmacz L, Swiezewska E (2011) Polyisoprenoids—secondary metabolites or physiologically important superlipids? Biochim Biophys Res Commun 407:627–632

    Article  CAS  Google Scholar 

  5. Skorupinska-Tudek K, Poznanski J, Wojcik J, Bienkowski T, Szostkiewicz I, Zelman-Femiak M, Bajda A, Chojnacki T, Olszowska O, Grunler J, Meyer O, Rohmer M, Danikiewicz W, Swiezewska E (2008) Contribution of the mevalonate and methylerythritol phosphate pathways to the biosynthesis of dolichols in plants. J Biol Chem 283:21024–21035

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  6. Schwarz F, Aebi M (2011) Mechanisms and principles of N-linked protein glycosylation. Curr Opin Struct Biol 21:576–582

    Article  PubMed  CAS  Google Scholar 

  7. Chojnacki T, Dallner G (1988) The biological role of dolichol. Biochem J 251:1–7

    PubMed Central  PubMed  CAS  Google Scholar 

  8. Bergamini E (2003) Dolichol: an essential part in the antioxidant machinery of cell membranes? Biogerontology 4:337–379

    Article  PubMed  CAS  Google Scholar 

  9. Bajda A, Konopka-Postupolska D, Krzymowska M, Hennig J, Skorupinska-Tudek K, Surmacz L, Wojcik J, Matysiak Z, Chojnacki T, Skorzynska-Polit E, Drazkiewicz M, Patrzylas P, Tomaszewska M, Kania M, Swist M, Danikiewicz W, Piotrowska W, Swiezewska E (2009) Role of polyisoprenoids in tobacco resistance against biotic stresses. Physiol Plant 135:351–364

    Article  PubMed  CAS  Google Scholar 

  10. Valtersson C, van Duijn G, Verkleij AJ, Chojnacki T, de Kruijff B, Dallner G (1985) The influence of dolichol, dolichyl esters, and dolichyl phosphate on phospholipid polymorphism and fluidity in model membranes. J Biol Chem 260:2742–2751

    PubMed  CAS  Google Scholar 

  11. Wang X, Mansourian AR, Quinn PJ (2008) The effect of dolichol on the structure and phase behaviour of phospholipid model membranes. Mol Membr Biol 25:547–556

    Article  PubMed  CAS  Google Scholar 

  12. Ciepichal E, Jemiola-Rzeminska M, Hertel J, Swiezewska E, Strzalka K (2011) Configuration of polyisoprenoids affects the permeability and thermotropic properties of phospholipid/polyisoprenoid model membranes. Chem Phys Lipids 164:300–306

    Article  PubMed  CAS  Google Scholar 

  13. Rezanka T, Votruba J (2001) Chromatography of long chain alcohols (polyprenols) from animal and plant sources. J Chromatogr A 936:95–110

    Article  PubMed  CAS  Google Scholar 

  14. Chojnacki T, Swiezewska E, Vogtman T (1987) Polyprenols from plants—structural analogues of mammalian dolichols. Chim Scripta 27:209–214

    CAS  Google Scholar 

  15. Skorupinska-Tudek K, Bienkowski T, Olszowska O, Furmanowa M, Chojnacki T, Danikiewicz W, Swiezewska E (2003) Divergent pattern of polyprenols and dolichols in different organs in Coluria geoides. Lipids 38:981–991

    Article  PubMed  CAS  Google Scholar 

  16. Kania M, Skorupinska-Tudek K, Swiezewska E, Danikiewicz W (2012) Atmospheric pressure photoionization mass spectrometry as a valuable method for the identification of polyisoprenoid alcohols. Rapid Commun Mass Spectrom 26:1705–1710

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This investigation was supported by a grant funded by the Polish National Cohesion Strategy Innovative Economy [UDA-POIG 01.03.01-14-036/09] and the School of Molecular Biology IBB PAS to K.G. We would like to thank Agata Lipko, IBB PAS for her help with TLC analyses.

Author information

Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

    Katarzyna Gawarecka & Ewa Swiezewska

Authors
  1. Katarzyna Gawarecka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ewa Swiezewska
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ewa Swiezewska .

Editor information

Editors and Affiliations

  1. Centre for Research in Agricultural Genomic (CRAG), CSIC-IRTA-UAB-UB, Barcelona, Spain

    Manuel Rodríguez-Concepción

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media New York

About this protocol

Cite this protocol

Gawarecka, K., Swiezewska, E. (2014). Analysis of Plant Polyisoprenoids. In: Rodríguez-Concepción, M. (eds) Plant Isoprenoids. Methods in Molecular Biology, vol 1153. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0606-2_9

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-0606-2_9

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0605-5

  • Online ISBN: 978-1-4939-0606-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation