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Analysis of Carotenoids and Tocopherols in Plant Matrices and Assessment of Their In Vitro Antioxidant Capacity

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Plant Isoprenoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1153))

Abstract

Carotenoids and tocopherols are lipid secondary metabolites that play essential roles in plants. They are also relevant compounds from a nutritional standpoint and attract much attention due to their proposed antioxidant properties. In this chapter, methodologies for the extraction and HPLC analysis of these compounds are described as well as a widely used protocol to assess their antioxidant capacity.

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Acknowledgements

A.J.M.M. acknowledges funding from the European Union (FP6-24671-QUALITOM and FP7-224789-INTERCAROT) and the Spanish programs Juan de la Cierva (JCI-2008-1767) and Ramón y Cajal (RYC-2010-07115). The authors are members of the IBERCAROT network, funded by CYTED (ref. 112RT0445).

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Authors and Affiliations

  1. Facultad de Farmacia, Department of Nutrition & Food Science, Food Colour & Quality Laboratory, Universidad de Sevilla, P. García González 2, 41012, Sevilla, Spain

    Antonio J. Meléndez-Martínez, Carla M. Stinco, Paula Mapelli Brahm & Isabel M. Vicario

Authors
  1. Antonio J. Meléndez-Martínez
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  2. Carla M. Stinco
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  3. Paula Mapelli Brahm
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  4. Isabel M. Vicario
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Corresponding author

Correspondence to Antonio J. Meléndez-Martínez .

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Editors and Affiliations

  1. Centre for Research in Agricultural Genomic (CRAG), CSIC-IRTA-UAB-UB, Barcelona, Spain

    Manuel Rodríguez-Concepción

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Meléndez-Martínez, A.J., Stinco, C.M., Brahm, P.M., Vicario, I.M. (2014). Analysis of Carotenoids and Tocopherols in Plant Matrices and Assessment of Their In Vitro Antioxidant Capacity. In: Rodríguez-Concepción, M. (eds) Plant Isoprenoids. Methods in Molecular Biology, vol 1153. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0606-2_6

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  • DOI: https://doi.org/10.1007/978-1-4939-0606-2_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0605-5

  • Online ISBN: 978-1-4939-0606-2

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